PMID: 9171420Jun 1, 1997Paper

Transcriptional regulation of delta-aminolevulinic acid dehydratase synthesis by oxygen in Bradyrhizobium japonicum and evidence for developmental control of the hemB gene

Journal of Bacteriology
S Chauhan, Mark R O'Brian

Abstract

An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein encoded by hemB were strongly and rapidly induced by O2 deprivation as determined by RNase protection and immunoblot analyses, but hemH message was not induced. Oxygen limitation resulted in a greater-than-10-fold increase in the rate of hemB mRNA synthesis as determined by transcriptional runoff experiments, whereas hemH transcription was unaffected by the O2 status. Thus, hemB is a regulated gene in B. japonicum and is transcriptionally controlled by O2. Unlike the expression in parent strain I110, hemB expression was not affected by O2 in the fixJ strain 7360, and O2-limited cultures of the mutant contained quantities of hemB mRNA and protein that were comparable to uninduced levels found in aerobic cells. In addition, spectroscopic analysis of cell extracts showed that increases in b- and c-type cytochromes and the disappearance of cytochrome aa3 in response to microaerobic growth in wild-type cells were not ob...Continue Reading

References

Sep 1, 1978·Journal of Bacteriology·Y J Avissar, K D Nadler
Jun 25, 1992·Nature·R F Fisher, S R Long
Jan 1, 1991·Annual Review of Biochemistry·J W Hershey
Jan 27, 1989·Cell·K PfeiferL Guarente
Jul 1, 1995·Journal of Bacteriology·K M Page, M L Guerinot
Mar 31, 1995·The Journal of Biological Chemistry·J M FrustaciM R O'Brian
Apr 1, 1995·Journal of Bacteriology·B A Schilke, T J Donohue
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·E SoupèneJ Batut
Jan 1, 1994·Antonie van Leeuwenhoek·J Batut, P Boistard
Sep 1, 1994·Microbiological Reviews·H M Fischer
Oct 1, 1993·Plant Molecular Biology·O MadsenK A Marcker
Aug 1, 1993·Applied and Environmental Microbiology·J M Frustaci, M R O'Brian
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O PreisigH Hennecke

❮ Previous
Next ❯

Citations

Aug 15, 1998·The Journal of Biological Chemistry·I HamzaM R O'Brian
Jan 27, 2017·Microbiology and Molecular Biology Reviews : MMBR·Harry A DaileyMartin J Warren
Jul 1, 2014·Molecular Microbiology·Thomas H Hohle, Mark R O'Brian
Mar 2, 2002·Journal of Bacteriology·Alan J BielKaren Sullivan
Feb 26, 2005·Infection and Immunity·Natalie D KingTerry D Connell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.