Transcriptional regulation of lung cytidylyltransferase in developing transgenic mice.

American Journal of Respiratory Cell and Molecular Biology
Diann M McCoyRama K Mallampalli

Abstract

Lung development is associated with a surge in surfactant phosphatidylcholine (PC) production to prepare the newborn for extrauterine breathing. This process is associated with a marked increase in the activity of the rate-regulatory surfactant enzyme, CTP:phosphocholine cytidylyltransferase (CCTalpha). To investigate the molecular basis for developmental activation of CCTalpha, we analyzed expression of endogenous CCTalpha and a reporter gene, beta-galactosidase, in fetal, newborn, and adult promoter-reporter transgenic mice. Transgenics harboring approximately 2 kb of the CCTalpha promoter linked upstream of a beta-galactosidase reporter gene displayed relatively high expression in distal lung epithelia. Endogenous lung CCTalpha and beta-galactosidase activities, protein content, and transcript levels displayed maximal expression within the newborn period. CCTalpha and beta-galactosidase activities and enzyme levels increased with time in cultured fetal lung explants isolated from transgenics. Transfectional analysis using CCTalpha promoter-reporter constructs in developing rat type II cells revealed that a region encompassing -169/+71 contained the DNA elements required for perinatal activation. The studies demonstrate that ...Continue Reading

References

Dec 21, 1977·Biochimica Et Biophysica Acta·V Oldenborg, L M Van Golde
Sep 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·I GrossS A Rooney
Aug 1, 1989·American Journal of Respiratory Cell and Molecular Biology·B E Miller, G E HooK
Nov 1, 1989·The Journal of Surgical Research·K T OldhamR R Wolfe
Feb 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·F ChanF Possmayer
Jul 1, 1994·The American Journal of Physiology·M HoganM Post
Sep 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A RooneyC R Mendelson
May 1, 1997·American Journal of Respiratory Cell and Molecular Biology·C A LongoR K Mallampalli
Jul 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M LiuG Enhorning
Dec 10, 1999·Pediatric Research·D M McCoyR K Mallampalli
Jan 21, 2000·Journal of Applied Physiology·R G SalomeR K Mallampalli
May 30, 2002·American Journal of Respiratory Cell and Molecular Biology·Jiali LiRoger G Spragg
Oct 23, 2002·Journal of Applied Physiology·Alan J RyanRama K Mallampalli
Jun 12, 2003·The European Respiratory Journal·K S BabuJ H Conway
Jun 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Jiming ZhouRama K Mallampalli
May 14, 2004·The Biochemical Journal·Alan J RyanRama K Mallampalli
Nov 13, 2004·The Journal of Biological Chemistry·Suzanne Jackowski, Paolo Fagone
Mar 25, 2005·The Journal of Biological Chemistry·Marianna AgassandianRama K Mallampalli

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Citations

Dec 19, 2009·The Journal of Biological Chemistry·Phillip L Butler, Rama K Mallampalli
Dec 17, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marianna AgassandianRama K Mallampalli
Apr 22, 2008·Biochimica Et Biophysica Acta·Hebe MarcucciClaudia Banchio
Feb 26, 2008·Progress in Lipid Research·Hiroyuki SugimotoDennis E Vance
Dec 15, 2006·Cellular Microbiology·Yanghong WuRama K Mallampalli
Oct 3, 2012·Biochimica Et Biophysica Acta·Marianna Agassandian, Rama K Mallampalli
Dec 4, 2012·Biochimica Et Biophysica Acta·Victoria GossAnthony D Postle
Feb 1, 2015·Cell and Tissue Research·Hans Gerd NothwangMarc A Willaredt
Oct 1, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Benno A GrzesikIstván Vadász

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