Transcriptional regulation of neuronal nicotinic acetylcholine receptor genes. Functional interactions between Sp1 and the rat beta4 subunit gene promoter.

The Journal of Biological Chemistry
C B BiggerP D Gardner

Abstract

To date, 11 members (alpha2-alpha9 and beta2-beta4) of the neuronal nicotinic acetylcholine receptor gene family have been identified. These genes encode subunits that form distinct receptors with different pharmacological and physiological profiles in temporally and spatially restricted patterns within the nervous system. Distinct molecular mechanisms probably orchestrate the expression of various receptor subtypes, yet little is known of specific transcriptional regulatory elements and their associated factors that are responsible for this segregated pattern of expression. Here we report the identification of an element, in the 5'-flanking region of the rat beta4 subunit gene, containing a CA box that is necessary for beta4 promoter activity in a transiently transfected cholinergic cell line, SN17. This element was shown to interact with a protein(s) in SN17 nuclear extracts that is antigenically related to the transcriptional activator Sp1. Furthermore, co-transfection experiments confirmed that Sp1 can transactivate a beta4 promoter-reporter gene construct, indicating that Sp1 is necessary, at least in part, for transcriptional activation of the beta4 subunit gene.

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Citations

Apr 20, 2000·European Journal of Pharmacology·I N MelnikovaP D Gardner
Oct 25, 2000·Neuropharmacology·I N MelnikovaP D Gardner
Oct 31, 2000·Gene·S D AndrewB G Robinson
Nov 14, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Linda A deGraffenriedSuzanne A W Fuqua
Apr 28, 2004·Breast Cancer Research and Treatment·Linda A deGraffenriedSuzanne A W Fuqua
Aug 23, 2000·Nucleic Acids Research·G L GalliaK Khalili
Apr 3, 2008·Neuroreport·Michael D ScofieldPaul D Gardner
Feb 4, 2010·Molecular Cancer Research : MCR·Ma Reina D ImprogoPaul D Gardner
May 17, 2007·The Journal of Biological Chemistry·Yuly F Fuentes Medel, Paul D Gardner
Nov 5, 1997·The Journal of Biological Chemistry·C B BiggerP D Gardner
Mar 23, 2001·The Journal of Biological Chemistry·I N Melnikova, P D Gardner
Dec 6, 2001·The Journal of Biological Chemistry·Nicole Francis, Evan S Deneris
Mar 30, 2001·The Journal of Biological Chemistry·U NagavarapuR T Boyd

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