Transcriptional regulation of prostaglandin synthase 2 gene expression by platelet-derived growth factor and serum.

The Journal of Biological Chemistry
W Xie, H R Herschman

Abstract

Prostaglandin synthase 2 (PGS2) is an immediate-early gene induced in a variety of cellular contexts. We investigate here the transcriptional activation of the murine PGS2 gene in NIH 3T3 cells, in response to the mitogens platelet-derived growth factor (PDGF) or serum. Site-directed mutagenesis experiments demonstrate that a consensus cyclic AMP response element (CRE) in the murine PGS2 promoter is essential for optimal PGS2 gene expression in response to PDGF or to serum. Overexpression of c-Jun potentiates PDGF- or serum-induced luciferase expression from a reporter construct containing the first 371 nucleotides of the PGS2 promoter. In contrast, overexpression of other transcription factors binding to the CRE element of the PGS2 gene inhibits induction by PDGF or serum. Moreover, positioning the c-Jun activation domain next to the minimal PGS2 promoter via a GAL4 DNA binding site rather than the CRE is sufficient to permit serum or PDGF stimulation of luciferase expression from this modified reporter construct. PDGF or serum treatment both activate c-Jun N-terminal kinase (JNK), the mitogen-activated protein kinase responsible for phosphorylation and activation of c-Jun. Cotransfection of plasmids expressing dominant-negati...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C C FranklinA S Kraft
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W L XieD L Simmons
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Apr 24, 1991·Biochimica Et Biophysica Acta·W L Smith, L J Marnett
May 8, 1991·Biochimica Et Biophysica Acta·D L DeWitt
Dec 1, 1988·Genes & Development·F J RauscherT Curran
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C BrunnerH J Müller
Aug 25, 1995·Cell·A B Vojtek, J A Cooper
Nov 1, 1994·Trends in Biochemical Sciences·R J Davis
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Nov 1, 1993·Molecular and Cellular Biology·S G MacdonaldF McCormick
Jan 5, 1996·Biochimica Et Biophysica Acta·H R Herschman
Mar 27, 1996·Biochemical and Biophysical Research Communications·M HayakawaF Ito
Dec 1, 1995·Molecular Reproduction and Development·R J Davis
Dec 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H R HerschmanS Reddy

❮ Previous
Next ❯

Citations

Oct 12, 2000·Biochemical and Biophysical Research Communications·L W SliceJ H Walsh
Jan 29, 2002·Journal of Molecular and Cellular Cardiology·Nicole ScheurenWinfried Schorb
Jun 6, 2012·Pflügers Archiv : European journal of physiology·Jurgen Schnermann, Josephine P Briggs
Jul 13, 2007·Breast Cancer Research and Treatment·Gurpreet Singh-RangerKefah Mokbel
Feb 27, 2004·Biochimica Et Biophysica Acta·Onno KranenburgEmile E Voest
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Tadashi Tanabe, Norimitsu Tohnai
Jan 25, 2003·Burns : Journal of the International Society for Burn Injuries·Martin G Schwacha
May 28, 2003·Journal of the American College of Cardiology·Orina Belton, Desmond Fitzgerald
Mar 10, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·F Marks, G Fürstenberger
Jan 1, 1998·Trends in Cardiovascular Medicine·J F Schmedtje, Y S Ji
May 1, 1998·Trends in Cardiovascular Medicine·H R Herschman
Jul 5, 2003·Biochimica Et Biophysica Acta·Yan J JiangPatrick C Choy
Sep 29, 2011·Chemical Reviews·William L SmithPer-Johan Jakobsson
Oct 12, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sabine KajitaRicardo V Lloyd
Oct 14, 2011·Nature Reviews. Cancer·Yuliya Pylayeva-GuptaDafna Bar-Sagi
Dec 1, 1999·British Journal of Pharmacology·J A Mitchell, T D Warner
Oct 14, 2004·European Journal of Biochemistry·Joanna E ChiversRobert Newton
Jul 9, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Inge CarlsenRikke Nørregaard
Sep 7, 2000·The Journal of Clinical Investigation·H F ChengR C Harris
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Daichi ChikazuCarol C Pilbeam
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·X M XuK K Wu
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dilprit BaggaSrinivasa T Reddy
Dec 21, 2007·Digestive Diseases and Sciences·Yan-Shi GuoMark R Hellmich
Mar 3, 2001·Neoplasia : an International Journal for Oncology Research·Z ZhangR N DuBois
Aug 6, 2010·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Rupal DevaSantosh Nigam
Aug 21, 2001·The Journal of Investigative Dermatology·H IhnM Trojanowska

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.