Transcriptional regulation of the assT-dsbL-dsbI gene cluster in Salmonella enterica serovar Typhi IMSS-1 depends on LeuO, H-NS, and specific growth conditions.

Journal of Bacteriology
A L Gallego-HernándezEdmundo Calva

Abstract

The assT gene encodes an arylsulfate sulfotransferase, an enzyme that catalyzes sulfuryl transfer from phenolic sulfate to a phenolic acceptor. In Salmonella enterica serovar Typhi IMSS-1, the assT gene is located upstream of the dsbL and dsbI genes, which are involved in a disulfide bond formation required for its activation. The assT-dsbL-dsbI gene cluster forms an operon transcribed by a LeuO-dependent promoter, in rich medium A (MA). Interestingly, in the absence of cloned leuO and in a ΔleuO background, two transcription start sites were detected for assT and two for dsbL-dsbI in minimal medium. The H-NS nucleoid protein repressed the expression of the assT-dsbL-dsbI LeuO-dependent operon, as well as of the assT transcriptional units. Thus, the expression of the assT-dsbL-dsbI gene cluster depends on the global regulatory proteins LeuO and H-NS, as well as on specific growth conditions.

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Citations

Jul 21, 2012·Molecular Microbiology·Ismael Hernández-Lucas, Edmundo Calva
Jul 19, 2012·Molecular Microbiology·Shane C DillonCharles J Dorman
Dec 21, 2013·Molecular Microbiology·Elena Espinosa, Josep Casadesús
Oct 1, 2021·Journal of Medical Microbiology·Marcos Fernández-MoraEdmundo Calva
Jan 11, 2022·Journal of Medical Microbiology·Isaac B Olivar-CasiqueIsmael Hernández-Lucas

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