Jun 16, 2015

Transcriptional regulation of the Chlamydia heat shock stress response in an intracellular infection

Molecular Microbiology
Brett R Hanson, Ming Tan


Bacteria encode heat shock proteins that aid in survival during stressful growth conditions. In addition, the major heat shock proteins of the intracellular bacterium Chlamydia trachomatis have been associated with immune pathology and disease. We developed a ChIP-qPCR method to study the regulation of chlamydial heat shock gene regulation during an intracellular infection. This approach allowed us to show that chlamydial heat shock genes are regulated by the transcription factor HrcA within an infected cell, providing validation for previous in vitro findings. Induction of chlamydial heat shock gene expression by elevated temperature was due to loss of HrcA binding to heat shock promoters, supporting a mechanism of derepression. This heat shock response was rapid, whereas recovery of HrcA binding and return to non-stress transcript levels occurred more slowly. We also found that control of heat shock gene expression was differentially regulated over the course of the intracellular Chlamydia infection. There was evidence of HrcA-mediated regulation of heat shock genes throughout the chlamydial developmental cycle, but the level of repression was lower at early times. This is the first study of Chlamydia-infected cells showing t...Continue Reading

  • References37
  • Citations8

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Infectious Agent Antigen Detection by Immunoassay With Direct Optical Observation; Chlamydia Trachomatis
Chlamydia Infections
Immune Response
Heat shock proteins
Biological Adaptation to Stress
Transcriptional Regulation
Chlamydial Pneumonia

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.