Transcriptional regulation of the heterocyst patterning gene patA from Anabaena sp. strain PCC 7120.

Journal of Bacteriology
Shirley S Young-RobbinsSean M Callahan

Abstract

The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucleotides -305, -614, and -645 relative to the translational start site (-305, -614, and -645 tsps). Transcriptional reporter fusions were used to show that transcription from the -305 tsp was induced in all cells of filaments in response to nitrogen deprivation, required hetR for induction, and increased in a patA mutant. Transcription from -614/-645 tsp reporter fusions was spatially regulated and occurred primarily in cells that would become heterocysts. Complementation of a patA mutant strain by alleles encoding substitutions in, or deletion of, the putative phosphoacceptor C-terminal domain indicates that the PATAN domain can function independently of the C-terminal domain of PatA. Localization of a ring of PatA-GFP at sites of cell division, as well as the formation of enlarged cells with altered cell morphology when patA was overexpressed, suggests that PatA may participate in cell division.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J LiangR Haselkorn
Jul 1, 1989·Journal of Bacteriology·M R Schaefer, S S Golden
Jan 1, 1988·Methods in Enzymology·J Elhai, C P Wolk
Oct 30, 1998·Science·H S Yoon, J W Golden
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W J Buikema, R Haselkorn
Jun 13, 2001·Molecular Microbiology·S M Callahan, W J Buikema
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xu HuangJindong Zhao
Feb 3, 2006·Journal of Bacteriology·Samer SakrTania Arcondeguy
Oct 7, 2008·Journal of Bacteriology·Douglas D Risser, Sean M Callahan

❮ Previous
Next ❯

Citations

Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Douglas D RisserJohn C Meeks
Jul 2, 2011·BMC Evolutionary Biology·John LarssonBirgitta Bergman
Oct 4, 2014·BMC Microbiology·Britt L FlahertyJames W Golden
Aug 18, 2012·Trends in Microbiology·Alicia M Muro-Pastor, Wolfgang R Hess
May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Javier Muñoz-García, Saúl Ares

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.