Transcriptional regulation of the human PRL-releasing peptide (PrRP) receptor gene by a dopamine 2 Receptor agonist: cloning and characterization of the human PrRP receptor gene and its promoter region

Molecular Endocrinology
Atsushi OzawaM Mori

Abstract

PRL-releasing peptide receptor (PrRPR) mRNA was expressed in pituitary adenomas but was not detected in patients treated with bromocriptine, a specific agonist of dopamine 2 (D2) receptor. Although medical treatment with bromocriptine is effective for patients with pituitary adenomas, little is known about the molecular mechanisms of gene regulation mediated by D2 receptors. The cloned human PrRPR gene spanned approximately 2.0 kb and contained two exons and one intron. Two functional polyadenylation signals located at 510 and 714 bp downstream from the stop codon. A primer extension analysis demonstrated two major transcriptional start sites at 139 and 140 bp upstream from the translational start site and an additional minor site at -161. The promoter region contained several putative binding sites for transcriptional factors including pituitary-specific transcription factor (Pit 1), activator protein 1 (AP-1), and specificity protein (Sp1), but no typical TATA or CAAT box. This promoter showed the strong activity in the pituitary-derived GH4C1 cells, and the region between -697 and -596 bp was responsible for the stimulation both by forskolin and overexpression of cAMP response element binding protein (CREB). These stimulatio...Continue Reading

References

May 1, 1992·Endocrine Reviews·J S BevanM F Scanlon
Dec 1, 1990·The Journal of Clinical Endocrinology and Metabolism·V HermanS Melmed
Aug 10, 1990·Science·J LyonsH R Bourne
Dec 22, 1988·Nature·J R BunzowO Civelli
Apr 17, 1995·Biochemical and Biophysical Research Communications·S K WelchH C Heller
Mar 1, 1994·European Journal of Biochemistry·O ValdenaireJ Mallet
Oct 28, 1993·Nature·J C ChriviaR H Goodman
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·H M ShihM F Hoekstra
Feb 11, 1998·Physiological Reviews·C MissaleM G Caron
Jun 2, 1998·Nature·S HinumaM Fujino
Jun 11, 1999·Biochemical and Biophysical Research Communications·H MatsumotoM Fujino
Sep 25, 1999·Regulatory Peptides·R FujiiM Fujino
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z YanP Greengard
Oct 19, 1999·Synapse·C H LammersM M Mouradian
Apr 25, 2000·Neuroendocrinology·H JarryK Bauer
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg
Oct 12, 2000·Biochemical and Biophysical Research Communications·M KishimotoK Chihara

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Citations

Nov 27, 2014·Frontiers in Endocrinology·Tetsuya Tachibana, Tatsuya Sakamoto

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