Transcriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells

The Biochemical Journal
V Camara-ClayetteJ P Cartron

Abstract

The Kell blood-group antigen was originally reported to be a protein expressed in erythroid tissue only. Transcriptional analysis of the KEL promoter activity in human erythroleukaemia K562 and epithelial HeLa cells by electrophoretic mobility-shift and supershift assays, chloramphenicol acetyltransferase assays, co-transfection studies and site-directed mutagenesis provided the following results: (i) the KEL promoter exhibits a strong transcriptional activity in K562 cells and, unexpectedly, a basal non-erythroid activity in HeLa cells, (ii) up-regulation of the 5' distal promoter activity occurs only in the erythroid context, and (iii) two motifs localized in the exon 1 region, which bind the Sp1/Sp3 and the human GATA-1/Ku70/80 factors, were required for down-regulation of the promoter activity, but inhibition of the promoter activity by the repressing factors in HeLa cells was incomplete. KEL expression in HeLa cells was performed further by primer-extension analysis, which revealed the presence of a low amount of Kell transcript correlating with basal expression of the Kell protein in these cells, as shown by immunopurification and Western-blot analysis. DNA sequencing of the transcript revealed a sequence identical to tha...Continue Reading

References

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Citations

Aug 14, 2001·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J P Cartron, Y Colin
Sep 19, 2007·BMC Molecular Biology·Raffaella Di LisiStefano Schiaffino
Apr 10, 2007·FEBS Letters·Alexander M BoutanaevDmitry I Nurminsky
Aug 29, 2013·Transfusion·Colvin M Redman, Soohee Lee
Sep 8, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Markus T RojewskiWilly A Flegel
Jul 30, 2002·The Journal of Biological Chemistry·David M WillisDwight A Towler
May 11, 2002·Leukemia & Lymphoma·T WagnerK Geissler

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