Transcriptional regulation of the mouse CSF-1 gene

Molecular Reproduction and Development
M A HarringtonA Song

Abstract

Research in our laboratory is aimed at understanding the cellular and molecular mechanisms that govern colony stimulating factor-1 (CSF-1) gene expression. Our hypothesis is that a basal set of trans-acting factors is bound to the CSF-1 gene during fibroblast proliferation, resulting in constitutive CSF-1 gene expression. Modulation of CSF-1 gene transcription by growth-arrest (decrease) or stimulation of growth-arrested fibroblasts (re-initiate) is mediated by changes in the basal set of factors bound and/or by the addition of stimulus-specific factors. We have extended our hypothesis to include other cell types (monocytes) to determine if mechanisms used to control CSF-1 gene expression in fibroblasts are unique or represent common nontissue-specific regulatory mechanisms. Analysis of CSF-1-CAT reporter constructs in transiently transfected fibroblasts and monocytes was used to identify CSF-1 genomic sequences that affect transcriptional activity. DNase I protection, electrophoretic mobility shift, and methylation interference assays were used to identify the putative cis-acting elements. Results of our study suggest multiple trans-acting factors may regulate CSF-1 gene expression; some may be tissue specific, while others, s...Continue Reading

References

Jan 1, 1992·Current Topics in Microbiology and Immunology·D A Rappolee, Z Werb
Aug 1, 1991·Genes & Development·J PelletierD Housman
Sep 1, 1990·Molecular and Cellular Biology·M A HarringtonH E Broxmeyer
Nov 1, 1995·DNA and Cell Biology·B W KonicekM A Harrington

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Citations

Jul 12, 2002·Journal of Cellular Biochemistry·Masako KanekiyoKeiichi Tanaka
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
May 17, 2000·Journal of Hematotherapy & Stem Cell Research·M Green, M A Harrington
Nov 4, 2015·Journal of Cellular Physiology·Yunyuan LiAziz Ghahary
Dec 10, 2008·Developmental and Comparative Immunology·Patrick C HaningtonMiodrag Belosevic
Dec 22, 2007·Journal of Dental Research·Y WittrantS Abboud-Werner
Jun 18, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sherry L AbboudKathleen Woodruff
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tiehui WangChristopher J Secombes

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