Transcriptional regulation of the nitrile hydratase gene cluster in Pseudomonas chlororaphis B23.

Journal of Bacteriology
Toshihide SakashitaMichihiko Kobayashi

Abstract

An enormous amount of nitrile hydratase (NHase) is inducibly produced by Pseudomonas chlororaphis B23 after addition of methacrylamide as the sole nitrogen source to a medium. The expression pattern of the P. chlororaphis B23 NHase gene cluster in response to addition of methacrylamide to the medium was investigated. Recently, we reported that the NHase gene cluster comprises seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA). Sequence analysis of the 1.5-kb region upstream of the oxdA gene revealed the presence of a 936-bp open reading frame (designated nhpR), which should encode a protein with a molecular mass of 35,098. The deduced amino acid sequence of the nhpR product showed similarity to the sequences of transcriptional regulators belonging to the XylS/AraC family. Although the transcription of the eight genes (nhpR, oxdA, amiA, nhpABC, nhpS, and acsA) in the NHase gene cluster was induced significantly in the P. chlororaphis B23 wild-type strain after addition of methacrylamide to the medium, transcription of these genes in the nhpR disruptant was not induced, demonstrating that nhpR codes for a positive transcriptional regulator in the NHase gene cluster. A reverse transcription-PCR experiment revealed that fiv...Continue Reading

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Citations

Apr 17, 2012·PloS One·Alan O MarronGiselle Walker
May 12, 2020·Frontiers in Bioengineering and Biotechnology·Zhongyi ChengZhemin Zhou
May 3, 2019·Applied Microbiology and Biotechnology·Varsha P Chhiba-GovindjeeDean Brady
Nov 10, 2020·The Journal of General and Applied Microbiology·Yoshiteru HashimotoMichihiko Kobayashi

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