PMID: 11606209Oct 19, 2001Paper

Transcriptional regulation of the ovine intestinal Na+/glucose cotransporter SGLT1 gene. Role of HNF-1 in glucose activation of promoter function

European Journal of Biochemistry
Steven VayroSoraya P Shirazi-Beechey

Abstract

Dietary sugars D-glucose and D-galactose are transported across the intestinal brush-border membrane by the Na+/glucose cotransporter, SGLT1. In various species studied, it has been shown that the activity, and expression, of intestinal SGLT1 is regulated by dietary sugars. We report in this paper that regulation of the intestinal SGLT1 gene by lumenal sugar is due, in part, to an increase in transcription. Using deletion analyses of the -66/+21-bp fragment, we have identified the minimal region of the ovine SGLT1 promoter able to support transcription. Site-directed mutagenesis of the hepatic nuclear factor-1 (HNF-1) consensus motif within this domain eliminates basal promoter function. In addition, we show direct evidence for glucose-induced activation of the -66/+21-bp promoter region. There is a co-ordinated decline in the abundance of ovine intestinal HNF-1 and SGLT1 transcripts during transition from preruminant to adult ruminant. This decline is recovered after glucose infusion of adult sheep intestine. Similarly, as shown using DNA mobility-shift assays, the intensity of the HNF-1-binding complex to the target promoter sequence decreases during maturation of the animal; this is restored after intestinal sugar infusion. ...Continue Reading

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Citations

Feb 22, 2012·Journal of Physiology and Biochemistry·M I Maldonado-CervantesF Martinez-Morales
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·E E ConnorC Li
Aug 6, 2003·European Journal of Biochemistry·Jane DyerSoraya P Shirazi-Beechey
Dec 15, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Biju Sam KamalamFrançoise Médale
Aug 10, 2005·The Journal of Surgical Research·Ali TavakkolizadehDavid B Rhoads
Nov 5, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R Sabino-SilvaU F Machado
Jun 22, 2006·American Journal of Physiology. Renal Physiology·Maike VeyhlHermann Koepsell
Mar 15, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ramesh KekudaUma Sundaram
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
May 6, 2019·Journal of Physiology and Biochemistry·Ma José FelicesMa Jesús Rodríguez-Yoldi
Jan 26, 2005·Biochemical Society Transactions·J DyerS P Shirazi-Beechey
Jun 22, 2007·American Journal of Physiology. Renal Physiology·Yu Jin LeeHo Jae Han
Aug 24, 2020·Pflügers Archiv : European journal of physiology·Hermann Koepsell
Jul 6, 2010·Journal of Molecular and Cellular Cardiology·Sanjay K BanerjeeFerhaan Ahmad

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