Transcriptional regulation of the rainbow trout CYP19a gene by FTZ-F1 homologue

The Journal of Steroid Biochemistry and Molecular Biology
Hiromi KandaN Takamatsu

Abstract

In rainbow trout, there are at least two CYP19 genes (CYP19a and CYP19b). They encode distinct P450arom isozymes that are differentially expressed in the ovary and brain. To understand the transcriptional regulation of the rainbow trout CYP19a (rtCYP19a) gene in the ovary, we isolated its 5'-flanking region. The presence of potential FTZ-F1-binding sites prompted us to isolate the cDNA encoding a rainbow trout FTZ-F1 homologue (rtFTZ-F1) and analyze its effect on the rtCYP19a gene transcriptional activity. RT-PCR analysis showed overlapping expression of the rtCYP19a and rtFTZ-F1 genes in the ovary. Transient transfection studies in Chinese hamster ovary-derived CHO-K1 cells revealed that the region from -247 to -105, which contains three potential FTZ-F1-binding sites, was required for rtFTZ-F1-mediated transcriptional activation of the rtCYP19a gene. Among the three potential binding sites, the two from -150 to -142 and from -118 to -110 showed strong affinities for rtFTZ-F1 in gel shift assays, and base substitutions in either site almost abolished the transcriptional activation by rtFTZ-F1. Taken together, these results demonstrate that rtFTZ-F1 plays an important role in the transcriptional regulation of the rtCYP19a gene ...Continue Reading

References

Jan 24, 1992·Science·A Elbrecht, R G Smith
Sep 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K ShinodaO Niwa
Sep 4, 1996·Biochemical and Biophysical Research Communications·K ObaH Nawata
May 24, 2000·The Journal of Steroid Biochemistry and Molecular Biology·L G GraddyR C Simmen
Oct 26, 2001·Biochemical and Biophysical Research Communications·Y KazetoJ M Trant
Dec 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·A TchoudakovaG V Callard
Apr 3, 2002·The Journal of Biological Chemistry·Colin D ClyneEvan R Simpson
Nov 14, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Luisa Dalla ValleLorenzo Colombo
Sep 16, 2003·Molecular and Cellular Endocrinology·Margaret M HinshelwoodCarole R Mendelson
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Serdar E BulunMakio Shozu
May 8, 2004·Trends in Cell Biology·Elisabeth FayardKristina Schoonjans

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Citations

Feb 21, 2013·Archives of Environmental Contamination and Toxicology·Sumana DasDilip Mukherjee
Apr 9, 2008·Zoological Science·Kayo YamadaMichihiko Ito
Nov 14, 2012·General and Comparative Endocrinology·Sumana Das, Dilip Mukherjee
Nov 10, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kristian R von SchalburgBen F Koop
Mar 18, 2009·General and Comparative Endocrinology·Yann GuiguenChing-Fong Chang
Nov 23, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lihong ZhangWeimin Zhang
May 29, 2014·Journal of Fish Biology·X W ChenZ Y Shi
Feb 9, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kristian R von SchalburgBen F Koop
May 27, 2014·General and Comparative Endocrinology·Yang ZhangWeimin Zhang
Oct 6, 2016·Fish Physiology and Biochemistry·Wei ZhangYan He
Nov 1, 2013·Biology of Reproduction·Rajini SreenivasanLászló Orbán
Feb 24, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masahiro NitaHideki Kawasaki

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