PMID: 9428683Jan 15, 1998Paper

Transcriptional regulation of the rat poly(ADP-ribose) polymerase gene by Sp1

European Journal of Biochemistry
M J BergeronS L Guérin

Abstract

Expression of the gene encoding poly(ADP-ribose) polymerase (PARP), although ubiquitous, nevertheless varies substantially between tissues. We have recently shown that Sp1 binds five distinct target sequences (US-1 and F1-F4) in the rat PARP (rPARP) gene promoter. Here we used deletion analyses and site-directed mutagenesis to address the regulatory function played by these Sp1 sites on the basal transcriptional activity directed by the rPARP promoter. Transfection experiments revealed that the most proximal Sp1 site is insufficient by itself to direct any promoter activity. In addition, a weak negative regulatory element was identified between positions -101 and -60. The rPARP promoter directed high levels of chloramphenicol acetyltransferase activity in Jurkat T-lymphoblastoid and Ltk- fibroblast cells but only moderate levels in pituitary GH4C1 and liver HTC cells, correlating with the amounts of PARP detected in these cells by western blot analysis. However, the reduced promoter efficiency in HTC and GH4C1 cells did not result from the lack of Sp1 activity in these cells but suggested that yet uncharacterized regulatory proteins might turn off PARP gene expression by binding negative regulatory elements from the rPARP promo...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 15, 1992·Cancer Genetics and Cytogenetics·I J Urumov, Y Manolova
Jul 1, 1992·DNA and Cell Biology·M C NehlsD A Brenner
Dec 12, 1990·European Journal of Biochemistry·Y YokoyamaY Yamamoto
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L Janson, U Pettersson
Nov 15, 1989·Biochemical and Biophysical Research Communications·K MatsumotoM Ui
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B W CherneyM E Smulson
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H M AlkhatibM E Smulson
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C M GormanB H Howard
Oct 20, 1995·The Journal of Biological Chemistry·G HagenG Suske
Mar 24, 1995·The Journal of Biological Chemistry·A D AdamsM A Thompson
Sep 1, 1994·Molecular and Cellular Biochemistry·S L OeiM Schweiger
Aug 1, 1993·Molecular and Cellular Biology·J A Knezetic, G Felsenfeld
May 24, 1996·The Journal of Biological Chemistry·A SlominskiS J Gaudet
Apr 1, 1996·Molecular and Cellular Biology·J KarlsederE Wintersberger
Jul 26, 1996·The Journal of Biological Chemistry·A KovarikJ Taylor-Papadimitriou

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Citations

May 18, 2000·International Journal of Cancer. Journal International Du Cancer·V A Soldatenkov, M Smulson
Nov 19, 2003·Biochemical and Biophysical Research Communications·Man-ching KuAkiko Hata
Jul 4, 2013·Acta Ophthalmologica·Nick J R Maycock, John Marshall
Sep 1, 2004·Experimental Eye Research·Caroline LupienChristian Salesse
Aug 3, 2005·Journal of Molecular Biology·Patrick J RochetteRégen Drouin
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xi WuJian-Ting Zhang
Nov 11, 2003·The Journal of Nutrition·Jacob B GriffinJanos Zempleni

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