Transcriptional regulation of two conceptus interferon tau genes expressed in Japanese black cattle during peri-implantation period.

PloS One
Toshihiro SakuraiKazuhiko Imakawa

Abstract

Interferon tau (IFNT), produced by the mononuclear trophectoderm, signals the process of maternal recognition of pregnancy in ruminants. However, its expression in vivo and its transcriptional regulation are not yet well characterized. Objectives of this study were to determine conceptus IFNT gene isoforms expressed in the bovine uterus and to identify differences in promoter sequences of IFNT genes that differ in their expression. RNA-seq data analysis of bovine conceptuses on days 17, 20, and 22 (day 0  =  day of estrus) detected the expression of two IFNT transcripts, IFNT1 and IFNTc1, which were indeed classified into the IFNT gene clade. RNA-seq and quantitative RT-PCR analyses also revealed that the expression levels of both IFNT mRNAs were highest on day 17, and then decreased on days 20 and 22. Bovine ear-derived fibroblast (EF) cells, a model system commonly used for bovine IFNT gene transcription study in this laboratory, were cotransfected with luciferase reporter constructs carrying upstream (positions -637 to +51) regions of IFNT1 or IFNTc1 gene and various transcription factor expression plasmids including CDX2, AP-1 (Jun) and ETS2. CDX2, either alone or with the other transcription factors, markedly increased luc...Continue Reading

References

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Citations

Feb 19, 2017·The Journal of Endocrinology·Kazuhiko ImakawaKazuya Kusama
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Toshihiko Ezashi, Kazuhiko Imakawa
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Alan D Ealy, Lydia K Wooldridge
May 16, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Kazuhiko ImakawaKazuya Kusama

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Datasets Mentioned

BETA
M60903
DRA000549
DAA26985.1
AAT97058.1

Methods Mentioned

BETA
PCR
RNA-seq
electrophoresis
transfection
Assay
than

Software Mentioned

SOLiD3
Ensemble
MAFFT suite
INS
Sequence Detection System
BLASTP
ProtTest3
Ensembl
RAxML

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