Transcriptional regulation of Vibrio cholerae hemagglutinin/protease by the cyclic AMP receptor protein and RpoS

Journal of Bacteriology
Anisia J Silva, Jorge A Benitez

Abstract

Vibrio cholerae secretes a Zn-dependent metalloprotease, hemagglutinin/protease (HA/protease), which is encoded by hapA and displays a broad range of potentially pathogenic activities. Production of HA/protease requires transcriptional activation by the quorum-sensing regulator HapR. In this study we demonstrate that transcription of hapA is growth phase dependent and specifically activated in the deceleration and stationary growth phases. Addition of glucose in these phases repressed hapA transcription by inducing V. cholerae to resume exponential growth, which in turn diminished the expression of a rpoS-lacZ transcriptional fusion. Contrary to a previous observation, we demonstrate that transcription of hapA requires the rpoS-encoded sigma(s) factor. The cyclic AMP (cAMP) receptor protein (CRP) strongly enhanced hapA transcription in the deceleration phase. Analysis of rpoS and hapR mRNA in isogenic CRP+ and CRP- strains suggested that CRP enhances the transcription of rpoS and hapR. Analysis of strains containing hapR-lacZ and hapA-lacZ fusions confirmed that hapA is transcribed in response to concurrent quorum-sensing and nutrient limitation stimuli. Mutations inactivating the stringent response regulator RelA and the HapR-...Continue Reading

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Citations

Mar 19, 2009·Medical Molecular Morphology·Hiroaki AokiKouichi Sano
Sep 30, 2005·Nature·Karina B Xavier, Bonnie L Bassler
Oct 20, 2009·Applied and Environmental Microbiology·Hiroaki Hasegawa, Claudia C Häse
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Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Karolis VaitkeviciusSun Nyunt Wai
Mar 23, 2006·Infection and Immunity·Anisia J SilvaJorge A Benitez
Mar 10, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jorge A Benitez, Anisia J Silva
Nov 17, 2009·FEMS Microbiology Letters·Syed Zafar SultanJorge A Benitez
Feb 6, 2016·PLoS Neglected Tropical Diseases·Anisia J Silva, Jorge A Benitez
Jun 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Justin CruiteF Jon Kull

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