PMID: 2813402Nov 1, 1989

Transcriptional regulatory regions of testis-specific PGK2 defined in transgenic mice

Proceedings of the National Academy of Sciences of the United States of America
M O RobinsonM I Simon

Abstract

The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5' region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5' to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding construct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.

References

Jan 1, 1995·Developmental Genetics·N B Hecht
Jan 1, 1995·Developmental Genetics·M C HofmannJ L Millán
May 4, 2001·Molecular Reproduction and Development·B BhullarF A van der Hoorn
Oct 1, 1994·Molecular Reproduction and Development·R C IannelloI Kola
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D J Wolgemuth, F Watrin
Jun 9, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Baisong LuColin E Bishop
Jun 3, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Meisha A MorelliPaula E Cohen
Nov 1, 1990·Developmental Biology·F A van der HoornS K Nordeen
Nov 1, 1992·Developmental Biology·J R McCarreyJ J Rossi
Jul 30, 1990·Biochimica Et Biophysica Acta·M TamaruY Nakanishi
May 7, 1992·Biochimica Et Biophysica Acta·J FitzgeraldH H Dahl
Nov 7, 1995·Biochimica Et Biophysica Acta·K M FlowersL S Jefferson
Feb 1, 1994·Molecular and Cellular Endocrinology·H IkeshimaT Takano
Dec 14, 1995·Biochimica Et Biophysica Acta·K MitaC Katagiri
Aug 2, 2003·Biochemical and Biophysical Research Communications·Henrikki SanttiJorma J Palvimo
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A van der Hoorn, H A Tarnasky
May 14, 2004·Biology of Reproduction·Christopher B GeyerJ R McCarrey
Aug 1, 2008·Biology of Reproduction·Sarah M KehoeNaohiro Terada
Dec 28, 2007·Cytogenetic and Genome Research·P GrzmilP Burfeind
Oct 17, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Qiao-Song ZhengYi-Xun Liu
Sep 4, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhangsheng YangJ R McCarrey
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Diego LorenzettiMonica J Justice
Jan 13, 2009·Genesis : the Journal of Genetics and Development·Cory R NicholasRenee A Reijo Pera

Citations

Nov 1, 1976·The Journal of Experimental Zoology·J L VandeBergP J Close
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J J PeschonR D Palmiter
Jan 1, 1974·Biochemical Genetics·L P KozakE M Eicher
Apr 1, 1969·Biochemical Genetics·E Beutler
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P SzaboM Siniscalco
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R P EricksonA Salkeld

Related Concepts

Benign Neoplasm of Testis
Genome
Neoplasm of Uncertain or Unknown Behavior of Testis
Phosphoglycerate KINASE
Transcription, Genetic
House mice
Gene Expression
Phosphoglycerate kinase, testis specific
Human gene
Genomics

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.