Transcriptional repressor NIR functions in the ribosome RNA processing of both 40S and 60S subunits.

PloS One
Jianguo WuYang Ke

Abstract

NIR was identified as an inhibitor of histone acetyltransferase and it represses transcriptional activation of p53. NIR is predominantly localized in the nucleolus and known as Noc2p, which is involved in the maturation of the 60S ribosomal subunit. However, how NIR functions in the nucleolus remains undetermined. In the nucleolus, a 47S ribosomal RNA precursor (pre-rRNA) is transcribed and processed to produce 18S, 5.8S and 28S rRNAs. The 18S rRNA is incorporated into the 40S ribosomal subunit, whereas the 28S and 5.8S rRNAs are incorporated into the 60S subunit. U3 small nucleolar RNA (snoRNA) directs 18S rRNA processing and U8 snoRNA mediates processing of 28S and 5.8 S rRNAs. Functional disruption of nucleolus often causes p53 activation to inhibit cell proliferation. Western blotting showed that NIR is ubiquitously expressed in different human cell lines. Knock-down of NIR by siRNA led to inhibition of the 18S, 28S and 5.8S rRNAs evaluated by pulse-chase experiment. Pre-rRNA particles (pre-rRNPs) were fractionated from the nucleus by sucrose gradient centrifugation and analysis of the pre-RNPs components showed that NIR existed in the pre-RNPs of both the 60S and 40S subunits and co-fractionated with 32S and 12S pre-rRNAs ...Continue Reading

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Citations

Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chi A MaAshish Jain
May 29, 2014·Cell Cycle·Xavier Deschênes-SimardGerardo Ferbeyre
Mar 2, 2018·Development·Delphine DuteilRoland Schüle

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Methods Mentioned

BETA
tandem affinity purification
confocal microscopy
immunoprecipitation
transfection
acetylation
histone acetylation
PCR
X-ray

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