Transcriptional responses of the marine green alga Micromonas pusilla and an infecting prasinovirus under different phosphate conditions

Environmental Microbiology
Charles BachyAlexandra Z Worden

Abstract

Prasinophytes are widespread marine algae for which responses to nutrient limitation and viral infection are not well understood. We studied the picoprasinophyte, Micromonas pusilla, grown under phosphate-replete (0.65 ± 0.07 d-1 ) and 10-fold lower (low)-phosphate (0.11 ± 0.04 d-1 ) conditions, and infected by the phycodnavirus MpV-SP1. Expression of 17% of Micromonas genes in uninfected cells differed by >1.5-fold (q < 0.01) between nutrient conditions, with genes for P-metabolism and the uniquely-enriched Sel1-like repeat (SLR) family having higher relative transcript abundances, while phospholipid-synthesis genes were lower in low-P than P-replete. Approximately 70% (P-replete) and 30% (low-P) of cells were lysed 24 h post-infection, and expression of ≤5.8% of host genes changed relative to uninfected treatments. Host genes for CAZymes and glycolysis were activated by infection, supporting importance in viral production, which was significantly lower in slower growing (low-P) hosts. All MpV-SP1 genes were expressed, and our analyses suggest responses to differing host-phosphate backgrounds involve few viral genes, while the temporal program of infection involves many more, and is largely independent of host-phosphate backgr...Continue Reading

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Citations

Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David M NeedhamAlexandra Z Worden
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·David M NeedhamAlexandra Z Worden
Sep 8, 2018·Viruses·Elena L HorasLutz Becks
Feb 26, 2019·Frontiers in Microbiology·Jan F Finke, Curtis A Suttle
Nov 7, 2019·Nature Reviews. Microbiology·Amy E ZimmermanMaureen L Coleman
Sep 12, 2018·Viruses·Bradford P TaylorMatthias G Fischer
Jun 30, 2019·Current Opinion in Biotechnology·Weiqi FuKourosh Salehi-Ashtiani

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