Transcriptional Shifts Highlight the Role of Nutrients in Harmful Brown Tide Dynamics

Frontiers in Microbiology
Louie L WurchSonya T Dyhrman

Abstract

Harmful algal blooms (HABs) threaten ecosystems and human health worldwide. Controlling nitrogen inputs to coastal waters is a common HAB management strategy, as nutrient concentrations often suggest coastal blooms are nitrogen-limited. However, defining best nutrient management practices is a long-standing challenge: in part, because of difficulties in directly tracking the nutritional physiology of harmful species in mixed communities. Using metatranscriptome sequencing and incubation experiments, we addressed this challenge by assaying the in situ physiological ecology of the ecosystem destructive alga, Aureococcus anophagefferens. Here we show that gene markers of phosphorus deficiency were expressed in situ, and modulated by the enrichment of phosphorus, which was consistent with the observed growth rate responses. These data demonstrate the importance of phosphorus in controlling brown-tide dynamics, suggesting that phosphorus, in addition to nitrogen, should be evaluated in the management and mitigation of these blooms. Given that nutrient concentrations alone were suggestive of a nitrogen-limited ecosystem, this study underscores the value of directly assaying harmful algae in situ for the development of management stra...Continue Reading

References

Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Sep 30, 2008·Applied and Environmental Microbiology·Beth A StaufferDavid A Caron
Feb 21, 2009·Science·Daniel J ConleyGene E Likens
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Oct 1, 2010·Environmental Microbiology·Louie L WurchSonya T Dyhrman
Jan 11, 2011·Biochemistry·Dan S Tawfik, Ronald E Viola
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher J GoblerIgor V Grigoriev
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Mar 31, 2012·Annual Review of Marine Science·Donald M AndersonGustaaf M Hallegraeff
Jun 12, 2012·Nucleic Acids Research·Marc LohseBjörn Usadel
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anna M MichalakMelissa A Zagorski
Sep 1, 2014·Journal of Environmental Management·Keith DavidsonGrigorios Moschonas
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Harriet AlexanderSonya T Dyhrman
Dec 10, 2016·The ISME Journal·Weida GongAdrian Marchetti
Jun 29, 2017·Nature Communications·Mohammad MoniruzzamanSteven W Wilhelm
Dec 1, 2008·Harmful Algae·J HeislerM Suddleson

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Citations

Apr 10, 2020·Frontiers in Microbiology·Eric R GannSteven W Wilhelm
Apr 12, 2020·Scientific Reports·Gabriel MetegnierMickael Le Gac
Jan 20, 2022·Archives of Microbiology·Atif Khurshid WaniJuliana Heloisa Pinê Américo-Pinheiro

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Datasets Mentioned

BETA
SRP072764
PRJNA315054

Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

CD
Analysis of Sequence Counts ( ASC )
FastQC
Bowtie2
Hit
ASC
Trinity
Trimmomatic

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