Transcriptional synergism between vitamin D-responsive elements in the rat 25-hydroxyvitamin D3 24-hydroxylase (CYP24) promoter.

The Journal of Biological Chemistry
D M KerryBrian K May

Abstract

Transcription of the CYP24 gene is induced by 1,25-(OH)2D3 through a vitamin D receptor-dependent process. The functional activities of three possible vitamin D response elements (VDREs), located on the antisense strand of the rat CYP24 promoter, were investigated by transient expression of native and mutant promoter constructs in COS-1, JTC-12, and ROS 17/2.8 cells. A putative VDRE with a half-site spacing of 6 base pairs at -249/-232 (VDRE-3) did not contribute to 1,25-(OH)2D3 induced expression in the native promoter, although activity has been reported when the element was fused to the heterologous thymidine kinase promoter. Two VDREs with half-site spacings of 3 base pairs at -150/-136 and -258/-244 (VDRE-1 and VDRE-2, respectively), showed transcriptional synergism in COS-1 cells when treated with 1,25-(OH)2D3 (10(-7) to 10(-11) M). The contribution of both VDREs was hormone-concentration dependent from 10(-10) to 10(-12) M, with VDRE-1 demonstrating greatest sensitivity to 1,25-(OH)2D3. Transactivation by VDRE-1 was always greater than VDRE-2, but the converse was observed for the binding of vitamin D receptor-retinoid X receptor complex by each VDRE in gel mobility shift assays. The synergy observed between VDRE-1 and V...Continue Reading

References

Jan 1, 1991·Annual Review of Nutrition·J W Pike
Jan 1, 1991·Methods in Enzymology·T A KunkelJ McClary
Jan 11, 1989·Nucleic Acids Research·L BuluwelaT H Rabbitts
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 1, 1983·Annual Review of Biochemistry·H F DeLuca, H K Schnoes
Jan 27, 1995·The Journal of Biological Chemistry·C ZieroldH F DeLuca
Nov 1, 1994·Nutrition Reviews·S S Hannah, A W Norman
Mar 3, 1995·The Journal of Biological Chemistry·P N MacDonaldR K DeLisle
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Oct 28, 1994·Biochemical and Biophysical Research Communications·C Rochette-EglyM P Gaub
Aug 15, 1994·Biochemical and Biophysical Research Communications·J P Kahlen, C Carlberg
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C ZieroldH F DeLuca
Feb 18, 1993·Nature·C CarlbergW Hunziker
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Aug 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J LemayM Gascon-Barré

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Citations

Mar 12, 2004·Biochemical Pharmacology·Franci A WeytsSylvia Christakos
Oct 18, 2000·Endocrinology and Metabolism Clinics of North America·M K Thomas, M B Demay
Mar 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·S MesserlianR St-Arnaud
Aug 5, 2000·Current Opinion in Nephrology and Hypertension·K SooyS Christakos
Jun 11, 2002·Annual Review of Nutrition·John L OmdahlBrian K May
Jun 26, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Liyong WangJames C Fleet
Apr 18, 2001·The Journal of Clinical Investigation·Y SakaiM B Demay
May 13, 2006·The Journal of Clinical Investigation·Changcheng ZhouKenneth E Thummel
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J RussellN J Koszewski
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Indra Ramasamy
Nov 20, 2012·Future Medicinal Chemistry·Atsushi KittakaTai C Chen
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·X F KongM F Holick
Jan 30, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Sylvia ChristakosQi Shen
Mar 27, 2015·International Journal of Molecular Sciences·Tomasz WasiewiczMichal A Zmijewski
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Susanna MiettinenTimo Ylikomi
Mar 15, 2012·Clinical Biochemistry·Paul H AndersonHoward A Morris
Mar 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Bo-Ying BaoYi-Fen Lee
Aug 12, 2006·The Journal of Investigative Dermatology·Michael BärMichael Meurer
Jun 26, 2007·Biochemical and Biophysical Research Communications·Amélie MoreauJean-Marc Pascussi
Aug 2, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yan LiuSylvia Christakos
Jul 13, 2006·Annals of the New York Academy of Sciences·Marie B Demay
May 17, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Min CuiJames C Fleet
Jul 5, 2005·Biochemical and Biophysical Research Communications·Luis M EstebanEdith M Gardiner
Jan 10, 2008·Biochemical and Biophysical Research Communications·Giana AngeloRichard J Wood
Jul 28, 2015·Cancers·Irene RussoMauro Alaibac
Jan 10, 2003·Journal of Cellular Biochemistry·S ChristakosX Peng
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marielle Gascon-BarréAntonio Nanci
Apr 17, 2002·The Journal of Biological Chemistry·Zhongjian XieDaniel D Bikle
Sep 9, 2006·Journal of Cellular Biochemistry·Anna KlopotJames C Fleet

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