PMID: 9188579Jul 1, 1997Paper

Transcriptional targeting of herpes simplex virus for cell-specific replication

Journal of Virology
Shin-Ichi MiyatakeS D Rabkin

Abstract

Tissue- or cell-specific targeting of vectors is critical to the success of gene therapy. We describe a novel approach to virus-mediated gene therapy, where viral replication and associated cytotoxicity are limited to a specific cell type by the regulated expression of an essential immediate-early viral gene product. This is illustrated with a herpes simplex virus type 1 (HSV-1) vector (G92A) whose growth is restricted to albumin-expressing cells. G92A was constructed by inserting an albumin enhancer/promoter-ICP4 transgene into the thymidine kinase gene of mutant HSV-1 d120, deleted for both copies of the ICP4 gene. This vector also contains the Escherichia coli lacZ gene under control of the thymidine kinase promoter, a viral early promoter, to permit easy detection of infected cells containing replicating vector. In the adult, albumin is expressed uniquely in the liver and in hepatocellular carcinoma and is transcriptionally regulated. The plaquing efficiency of G92A is > 10(3) times higher on human hepatoma cells than on non-albumin-expressing human cells. The growth kinetics of G92A in albumin-expressing cells is delayed compared with that of wild-type HSV-1, likely due to aberrant expression of ICP4 protein. Cells undergo...Continue Reading

References

May 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Okuda
Mar 21, 1992·Lancet·A A GutierrezK Sikora
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B E HuberT A Krenitsky
May 1, 1989·Journal of Virology·B Panning, J R Smiley
Aug 1, 1989·Antimicrobial Agents and Chemotherapy·D M CoenS K Weller
Nov 1, 1989·Molecular and Cellular Biology·P HerbomelM C Weiss
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S HerbstL E Babiss
Sep 1, 1985·Journal of Virology·W R SacksP A Schaffer
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I H Gelman, S Silverstein
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P J Godowski, D M Knipe
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P BeardL I Pizer
Jun 11, 1987·Nucleic Acids Research·N A DeLuca, P A Schaffer
Jan 1, 1987·Methods in Enzymology·G J Darlington
Sep 1, 1974·The Journal of General Virology·A T JamiesonJ H Subak-Sharpe
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M Tilghman, A Belayew

❮ Previous
Next ❯

Citations

Apr 19, 2002·The Oncologist·John T Mullen, Kenneth K Tanabe
Jan 11, 2003·Cancer Gene Therapy·Susan Varghese, Samuel D Rabkin
Apr 22, 2003·Human Cell·Shin-Ichi Miyatake
Feb 11, 2004·Endocrine Reviews·Luisa BarzonGiorgio Palù
Apr 11, 2006·Cancer Gene Therapy·Y Shen, J Nemunaitis
Dec 13, 1997·Nature Medicine·R L Martuza
Oct 9, 2004·Cancer Gene Therapy·Bart Everts, Henk G van der Poel
Aug 3, 2004·Cancer Gene Therapy·Eugene Lin, John Nemunaitis
Jul 12, 2012·Nature Biotechnology·Stephen J RussellJohn C Bell
Feb 14, 2002·The Journal of General Virology·Christopher J A Ring
Oct 9, 2002·Annals of Surgery·John T MullenKenneth K Tanabe
Oct 19, 2005·Gene Therapy·R ArgnaniR Manservigi
Jul 21, 2005·Technology in Cancer Research & Treatment·Masato Yamamoto, David T Curiel
Oct 20, 2007·Orvosi hetilap·Zsolt Fábián
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·T TodoP A Johnson
Apr 1, 2009·Head & Neck Oncology·Edward J Shillitoe
Jan 22, 2015·Viruses·Md Zahidul Islam Pranjol, Amin Hajitou
Nov 19, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Rafaela ArgnaniRuben Hernandez-Alcoceba
Apr 5, 2000·The Journal of Clinical Investigation·R L Martuza
Jan 7, 2018·Journal of Cancer Research and Clinical Oncology·Weihua LouNing Zhang
Nov 25, 2020·Expert Opinion on Drug Discovery·Shunchuan Zhang, Samuel D Rabkin
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasushi InoTomoki Todo
Oct 25, 2000·The Journal of Gene Medicine·U HerrlingerS A Reeves
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·K NakanoM Tanaka
Mar 10, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·C G MillerN W Fraser
Nov 10, 2000·Advanced Drug Delivery Reviews·K J HarringtonR G Vile

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.