Transcriptional up-regulation of a novel ferritin homolog in abalone Haliotis discus hannai Ino by dietary iron

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Chenglong WuZhiguo Liufu

Abstract

A novel cDNA encoding ferritin (HdhNFT) was cloned from the hepatopancreas of abalone, Haliotis discus hannai Ino. The deduced protein contains 171 amino acid residues with a predicted molecular mass (MW) about 19.8 kDa and theoretical isoelectric point (pI) of 4.792. Amino acid alignment revealed that HdhNFT shared high similarity with other known ferritins. The HdhNFT contained a highly conserved motif for the ferroxidase center, which consists of seven residues of a typical vertebrate heavy-chain ferritin with a typical stem-loop structure. HdhNFT mRNA contains a 27 bp iron-responsive element (IRE) in the 5'-untranslated region. This IRE exhibited 82.14% similarity with abalone H. discus discus and 78.57% similarity with Pacific oyster Crassostrea gigas IREs. By real-time PCR assays, the mRNA transcripts of HdhNFT were found to be higher expressed in kidney, hepatopancreas, gill, mantle and muscle than in haemocytes and gonad. Moreover, mRNA expression levels of HdhNFT in the hepatopancreas and haemocytes were measured by real-time PCR in abalone fed with graded levels of dietary iron (29.2, 65.7, 1267.2 and 6264.7 mg/kg). Results showed that the expression of the HdhNFT mRNA increased with dietary iron contents. Furthermore...Continue Reading

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Citations

May 15, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo ZhuHe-Qing Huang
Mar 3, 2011·Fish & Shellfish Immunology·Chenghua LiTaiwu Li
Apr 5, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Teodoro Coba de la PeñaFederico M Winkler
Nov 30, 2019·Genes & Genomics·Jennifer Im, Heui-Soo Kim

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