Transcriptional up-regulation of disk abalone selenium dependent glutathione peroxidase by H(2)O(2) oxidative stress and Vibrio alginolyticus bacterial infection

Fish & Shellfish Immunology
Mahanama De ZoysaJehee Lee

Abstract

Selenium dependent glutathione peroxidase (Se-GPx) belongs to the family of selenoprotein, which acts mainly as an antioxidant in the cellular defence system. We have identified Se-GPx full length cDNA from disk abalone (Haliotis discus discus) designated as AbSe-GPx. It has a characteristic codon at (223)TGA(225) that corresponds to selenocysteine (Sec) amino acid as U(75). The full length cDNA consists of 675 bp, an open reading frame encoding 225 amino acids. Sequence characterization revealed that AbSe-GPx contains a characteristic GPx signature motif 2 ((97)LGFPCNQF(104)), an active site motif ((183)WNFEKF(188)) and essential residues for the enzymatic function. Additionally, the eukaryotic selenocysteine insertion sequence (SECIS) is conserved in the 3' UTR. The AbSe-GPx amino acid sequence exhibited the highest level of identity (46%) with insect (Ixodes scapularis) GPx, and shares 41% with bivalve (Unio tumidus) Se-GPx. The RT-PCR analysis revealed that AbSe-GPx mRNA was expressed constitutively in gill, mantle, gonad, abductor muscle, digestive tract, and hemocytes in a tissue specific manner. AbSe-GPx mRNA expression was significantly up-regulated in gill and digestive tract tissues after H(2)O(2) injection and Vibrio...Continue Reading

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Citations

Oct 8, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bao-Qing HuZhi-Ying Tao
May 9, 2012·Fish & Shellfish Immunology·Jesu ArockiarajSubha Bhassu
Jul 6, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Laura CanesiGabriella Gallo
Jul 6, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Changkao MuMing Cong
Nov 4, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Antonio García-TrianaGloria Yepiz-Plascencia
Aug 25, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mahanama De ZoysaJehee Lee
Aug 20, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Qiang WanJehee Lee
Feb 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhongguo ShanYunfeng Li
Nov 3, 2017·Chemical & Pharmaceutical Bulletin·Sakura YoshidaMorio Nakayama

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