Transcriptional up-regulation of the protooncogenes c-fos and c-jun following vitreous removal and short-term in vitro culture of bovine lenses

Experimental Eye Research
C ChiapponiS Ottonello

Abstract

Chemical (mainly oxidative) and mechanical (anterior capsule injury) stresses have been reported to up-regulate the expression of the protooncogenes c-fos and c-jun in the lens. Another potentially stressful, yet largely unexplored condition, inherent to all experiments requiring the in vitro culturing of isolated lenses, is vitreous removal. Based on the results of an extensive RNA gel blot analysis conducted on epithelial/capsule preparations isolated from calf lenses dissected and cultured under different conditions, we show, here, that lens isolation and short-term culture (1-2.5 hr, without any significant GSH depletion) result in a strong and time-dependent up-regulation of the c-jun and c-fos mRNAs. This response, which relies on transcriptional protooncogene activation and is more intense for c-fos than for c-jun, is in part prevented by the preservation of the lens-vitreous contact, but not by the culture of vitreous-stripped lenses on a vitreous bed. Supplementation of the culture medium with the antioxidant N -acetyl-cysteine slightly reduced the c-jun, but not the c-fos response. Protooncogene up-regulation thus appears to be mainly determined by the disruption of critical lens-vitreous interactions. Since this resp...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
Jun 1, 1992·Current Opinion in Cell Biology·L D KerrI M Verma
Jan 1, 1996·Free Radical Biology & Medicine·W C Li, A Spector
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Citations

Jul 26, 2005·Journal of Cataract and Refractive Surgery·Kumi ShiraiYoshitaka Ohnishi
Sep 5, 2003·Journal of Cataract and Refractive Surgery·Kumi ShiraiYoshitaka Ohnishi
Apr 7, 2010·Molecular Biology Reports·Sheng PengBingxu Ren

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