Transcriptome Analyses in Different Cucumber Cultivars Provide Novel Insights into Drought Stress Responses

International Journal of Molecular Sciences
Min WangYu'e Lin

Abstract

Drought stress is one of the most serious threats to cucumber quality and yield. To gain a good understanding of the molecular mechanism upon water deficiency, we compared and analyzed the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, L-9 (drought-tolerant) and A-16 (drought-sensitive). In our present study, combining the analysis of phenotype, twelve samples of cucumber were carried out a transcriptomic profile by RNA-Seq under normal and water-deficiency conditions, respectively. A total of 1008 transcripts were differentially expressed under normal conditions (466 up-regulated and 542 down-regulated) and 2265 transcripts under drought stress (979 up-regulated and 1286 down-regulated). The significant positive correlation between RNA sequencing data and a qRT-PCR analysis supported the results found. Differentially expressed genes (DEGs) involved in metabolic pathway and biosynthesis of secondary metabolism were significantly changed after drought stress. Several genes, which were related to sucrose biosynthesis (Csa3G784370 and Csa3G149890) and abscisic acid (ABA) signal transduction (Csa4M361820 and Csa6M382950), were specifically induced after 4 days of drought stress. DEGs between th...Continue Reading

References

Feb 14, 2002·Plant, Cell & Environment·L. Xiong, J.-K. Zhu
Jul 1, 1995·The Plant Cell·H. J. BohnertR. G. Jensen
Jun 19, 2004·Journal of Plant Physiology·Zhimin YinStefan Malepszy
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Jul 27, 2006·The New Phytologist·Tom Shepherd, D Wynne Griffiths
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Honghong HuLizhong Xiong
Nov 1, 2006·Journal of Experimental Botany·Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
May 1, 2007·Current Opinion in Plant Biology·Motoaki SekiKazuo Shinozaki
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
May 31, 2008·Comptes rendus biologies·Hong-bo ShaoHong-mei Mi
Dec 17, 2008·Plant Molecular Biology·Rajendra Bari, Jonathan D G Jones
Jan 13, 2009·Biochemical and Biophysical Research Communications·Xingnan ZhengBin Han
Nov 3, 2009·Nature Genetics·Sanwen HuangSonggang Li
Mar 19, 2011·Journal of Plant Research·Yasunari FujitaKazuko Yamaguchi-Shinozaki
Mar 29, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Julia Yue CuiCurtis D Klaassen
Oct 30, 2012·Progress in Lipid Research·Amélie Bernard, Jérôme Joubès
Oct 5, 2013·Biotechnology Advances·Agyemang DanquahHeribert Hirt
Jul 15, 2015·International Journal of Molecular Sciences·Chae Woo LimSung Chul Lee
Nov 30, 2016·Clinical Epigenetics·Tobias FehlmannAndreas Keller
Dec 3, 2017·Biochemical and Biophysical Research Communications·Kui HuangJianmei Wang

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Citations

Jun 4, 2019·International Journal of Molecular Sciences·Pedro Martínez-Gómez
Jun 3, 2020·Functional Plant Biology : FPB·Min WangYu E Lin
May 22, 2019·Frontiers in Plant Science·Priyanka TrivediKatja Karppinen

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Methods Mentioned

BETA
transgenic
RNA-seq
transmission electron microscopy
scanning electron microscopy
MDA
PCR

Software Mentioned

MEGA6
WEGO
NOISeq
GraphPad Prism
linux rhel6
SPSS Statistics

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