Transcriptome Analyses of Diaphorina citri Midgut Responses to Candidatus Liberibacter Asiaticus Infection

Insects
Hai-Zhong YuZhan-Jun Lu

Abstract

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important transmission vector of the citrus greening disease Candidatus Liberibacter asiaticus (CLas). The D. citri midgut exhibits an important tissue barrier against CLas infection. However, the molecular mechanism of the midgut response to CLas infection has not been comprehensively elucidated. In this study, we identified 778 differentially expressed genes (DEGs) in the midgut upon CLas infection, by comparative transcriptome analyses, including 499 upregulated DEGs and 279 downregulated DEGs. Functional annotation analysis showed that these DEGs were associated with ubiquitination, the immune response, the ribosome, endocytosis, the cytoskeleton and insecticide resistance. KEGG enrichment analysis revealed that most of the DEGs were primarily involved in endocytosis and the ribosome. A total of fourteen DEG functions were further validated by reverse transcription quantitative PCR (RT-qPCR). This study will contribute to our understanding of the molecular interaction between CLas and D. citri.

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Citations

Aug 25, 2020·Molecular Plant·Weijie HuangGitta Coaker
Apr 24, 2021·Journal of Proteome Research·Saeed HosseinzadehMichelle Heck

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Datasets Mentioned

BETA
SRX7026388
SRX7026389

Methods Mentioned

BETA
electrophoresis
PCR
ubiquitination
CLIP
GTPases

Software Mentioned

DESeq2
Hisat2
cBot Cluster Generation System
Genesis
Perl scripts
house
clusterProfiler R package
DESeq2 R
CASAVA

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