Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.)

Frontiers in Plant Science
Rui DongZhipeng Liu

Abstract

The seed dispersion caused by pod shattering is a form of propagation used by many wild species. Loss of seeds from pod shattering is frequent in the common vetch (Vicia sativa L.), an important self-pollinating annual forage legume. However, pod shattering is one of the most important defects that limits the reproduction of the vetch in the field and the usage as a leguminous forage crop. To better understand the vetch pod shattering mechanism, we used high-throughput RNA sequencing to assess the global changes in the transcriptomes of the pod ventral sutures of shattering-susceptible and shattering-resistant vetch accessions screened from 541 vetch germplasms. A total of 1,285 significantly differentially expressed unigenes (DEGs) were detected, including 575 up-regulated unigenes and 710 down-regulated unigenes. Analyses of Gene Ontology and KEGG metabolic enrichment pathways of 1,285 DEGs indicated that 22 DEGs encoding cell wall modifications and hydrolases associated with pod shattering were highly expressed in shattering-susceptible accessions. These genes were mainly enriched in "hydrolase activity," "cytoplasm," and "carbohydrate metabolic process" systems. These cell wall modifications and hydrolases genes included β-...Continue Reading

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Citations

Oct 18, 2018·International Journal of Molecular Sciences·Qiang ZhouZhipeng Liu
Feb 15, 2020·BMC Plant Biology·Lucía De la RosaElena Ramirez-Parra
Mar 21, 2020·Frontiers in Plant Science·Lisa Kissing KucekNick P Wiering
Oct 21, 2020·Scientific Reports·David Aguilar-BenitezAna M Torres

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Methods Mentioned

BETA
RNA Assay
PCR
RNA-Seq

Software Mentioned

FASTX
agriGO
Primer Express
HMMER
Blast2GO
STEM
topGO
Trinity Program
WEGO
Bowtie

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