Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis.

Scientific Reports
Thales Henrique Cherubino RibeiroAntonio Chalfun-Junior

Abstract

Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, whi...Continue Reading

References

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Aug 28, 2007·Nature·Olivier JaillonUNKNOWN French-Italian Public Consortium for Grapevine Genome Characterization
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Dec 3, 2016·Nucleic Acids Research·UNKNOWN NCBI Resource Coordinators

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Citations

May 6, 2021·International Journal of Molecular Sciences·Oluwaseun Olayemi AlukoHaobao Liu

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Datasets Mentioned

BETA
PRJNA558999

Methods Mentioned

BETA
dissection
RNA-seq

Software Mentioned

R
Blast2GO
edgeR
Trimmomatic
Trinity
TransDecoder
STAR
BlastKOALA
RSEM
htseq

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