Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.)

PeerJ
Dujun WangZhen Ouyang

Abstract

Mulberry (Morus alba L.) represents one of the most commonly utilized plants in traditional medicine and as a nutritional plant used worldwide. The polyhydroxylated alkaloid 1-deoxynojirimycin (DNJ) is the major bioactive compounds of mulberry in treating diabetes. However, the DNJ content in mulberry is very low. Therefore, identification of key genes involved in DNJ alkaloid biosynthesis will provide a basis for the further analysis of its biosynthetic pathway and ultimately for the realization of synthetic biological production. Here, two cDNA libraries of mulberry leaf samples with different DNJ contents were constructed. Approximately 16 Gb raw RNA-Seq data was generated and de novo assembled into 112,481 transcripts, with an average length of 766 bp and an N50 value of 1,392. Subsequently, all unigenes were annotated based on nine public databases; 11,318 transcripts were found to be significantly differentially regulated. A total of 38 unique candidate genes were identified as being involved in DNJ alkaloid biosynthesis in mulberry, and nine unique genes had significantly different expression. Three key transcripts of DNJ biosynthesis were identified and further characterized using RT-PCR; they were assigned to lysine de...Continue Reading

References

Dec 1, 1988·Journal of General Microbiology·J CremerH Sahm
Jan 1, 1985·Critical Reviews in Microbiology·J K Bhattacharjee
Jul 14, 1995·The Journal of Biological Chemistry·A J Tipping, M J McPherson
Jun 4, 1998·Journal of Natural Products·N AsanoG W Fleet
Dec 30, 1998·FEMS Microbiology Letters·T Kosuge, T Hoshino
Jul 27, 1999·Planta medica·H S KimJ J Lee
Jul 25, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 1, 1995·The Plant Cell·G. Galili
Aug 5, 2003·Uchū Seibutsu Kagaku·Tsukasa Iwashina
Oct 3, 2003·International Journal of Food Sciences and Nutrition·Sarita SrivastavaR P Srivastava
Jan 18, 2005·Biochimica Et Biophysica Acta·Andre' O HudsonThomas Leustek
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kotaro KonnoKatsuyuki Kohno
Jan 1, 1944·The Biochemical Journal·E F Gale, H M Epps
Jun 9, 2007·Journal of Agricultural and Food Chemistry·Toshiyuki KimuraTeruo Miyazawa
Jul 28, 2009·Journal of Agricultural and Food Chemistry·Li-Kaung LiuChau-Jong Wang
Apr 1, 2010·Biochimie·Tatiana P Soares da CostaJuliet A Gerrard
Oct 12, 2010·Science·Parayil Kumaran AjikumarGregory Stephanopoulos
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Sep 8, 2011·Nature Reviews. Genetics·Jeffrey A Martin, Zhong Wang
Jan 28, 2012·Journal of the American Chemical Society·Yongjin J ZhouZongbao K Zhao
Aug 21, 2012·Bioorganic & Medicinal Chemistry Letters·Jieyin SunIkuro Abe
Dec 20, 2013·Foodborne Pathogens and Disease·Ji-Hye LeeMi Sook Chung
May 16, 2017·Journal of Proteome Research·Dandan WangQingyou Xia

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
RNA-Seq
PCR
RNAseq

Software Mentioned

BlastX
SWISS
PROT
edgeR
cutadapt
rpsBlast
BLAST
Prinseq
SPSS
Primer Premier

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.