Transcriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp. creticus

Plant Molecular Biology
Vasiliki FalaraAngelos K Kanellis

Abstract

Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from different developmental stages revealed that young leaves of 1-2 cm length displayed the highest content of labdane-type diterpenes (80 mg/g fresh weight) whereas trichomes from older leaves (2-3 or 3-4 cm) exhibited gradual decreased concentrations. A cDNA library was constructed enriched in transcripts from trichomes isolated from young leaves, which are characterized by high levels of labdane-type diterpenes. Functional annotation of 2,022 expressed sequence tags (ESTs) from the trichome cDNA library based on homology to A. thaliana genes suggested that 8% of the putative identified sequences were secondary metabolism-related and involved primarily in flavonoid and terpenoid biosynthesis. A significant proportion of the ESTs (38%) displayed no significant similarity to any other DNA deposited in databases, indicating a yet unknown function. Custom DNA microarrays constructed with 1,248 individual clones from th...Continue Reading

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Citations

Nov 27, 2009·Plant Physiology·Xinbin DaiPatrick Xuechun Zhao
Feb 2, 2011·PloS One·Xiaodong BaiOmprakash Mittapalli
Dec 27, 2011·Progress in Lipid Research·Andréa HemmerlinThomas J Bach
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Sep 1, 2015·Frontiers in Plant Science·Konstantinos KoudounasPolydefkis Hatzopoulos
Sep 16, 2016·Plant Molecular Biology·Eugenia IoannidiAngelos K Kanellis
Oct 22, 2009·Natural Product Reports·Keiko Yonekura-Sakakibara, Kazuki Saito

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