Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes in leaves and fruits

Molecular Genetics and Genomics : MGG
Priscila Mary YuyamaLuiz Filipe Protasio Pereira

Abstract

Studies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next-generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differential...Continue Reading

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Citations

Aug 25, 2017·International Journal of Molecular Sciences·Long ZhangYuling Li
Sep 10, 2018·Functional & Integrative Genomics·Tiago Benedito Dos SantosDouglas S Domingues
Dec 9, 2020·International Journal of Molecular Sciences·Isabel MarquesAna I Ribeiro-Barros

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