Transcriptome analysis in rat kidneys: importance of genes involved in programmed hypertension

International Journal of Molecular Sciences
You-Lin TainChien-Te Lee

Abstract

Suboptimal conditions in pregnancy can elicit long-term effects on the health of offspring. The most common outcome is programmed hypertension. We examined whether there are common genes and pathways in the kidney are responsible for generating programmed hypertension among three different models using next generation RNA sequencing (RNA-Seq) technology. Pregnant Sprague-Dawley rats received dexamethasone (DEX, 0.1 mg/kg) from gestational day 16 to 22, 60% high-fructose (HF) diet, or NG-nitro-l-arginine-methyester (l-NAME, 60 mg/kg/day) to conduct DEX, HF, or l-NAME model respectively. All three models elicited programmed hypertension in adult male offspring. We observed five shared genes (Bcl6, Dmrtc1c, Egr1, Inmt, and Olr1668) among three different models. The identified differential genes (DEGs) that are related to regulation of blood pressure included Aqp2, Ptgs1, Eph2x, Hba-a2, Apln, Guca2b, Hmox1, and Npy. RNA-Seq identified genes in arachidonic acid metabolism are potentially gatekeeper genes contributing to programmed hypertension. In addition, HF and DEX increased expression and activity of soluble epoxide hydrolase (Ephx2 gene encoding protein). Conclusively, the DEGs in arachidonic acid metabolism are potentially gat...Continue Reading

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Citations

Dec 30, 2015·International Journal of Molecular Sciences·You-Lin Tain, Jaap A Joles
May 20, 2016·Intractable & Rare Diseases Research·Jing TianGuosheng Jiang
Feb 18, 2017·International Journal of Molecular Sciences·You-Lin Tain, Chien-Ning Hsu
Sep 26, 2017·Clinical and Translational Science·Ana Caroline C SáJulie A Johnson
Mar 16, 2019·Physiological Genomics·Elizabeth A SwansonDavid H Ellison
Feb 15, 2020·International Journal of Molecular Sciences·Verónica Guarner-LansIsrael Pérez-Torres
Jun 18, 2018·Current Hypertension Reports·Olga E Redina, Arcady L Markel
Mar 25, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Si-Ming MaCun-Zhi Liu
Apr 29, 2020·Journal of Clinical Medicine·Wen-Chin LeeChien-Te Lee
Feb 16, 2019·International Journal of Molecular Sciences·Chien-Ning Hsu, You-Lin Tain
Jan 29, 2019·Frontiers in Pharmacology·Jun-Yan Liu
Nov 27, 2019·International Journal of Molecular Sciences·Chien-Ning HsuYou-Lin Tain
Mar 22, 2019·Nature Reviews. Cardiology·Laura CalvilloGianfranco Parati
Mar 27, 2020·International Journal of Molecular Sciences·Chien-Ning Hsu, You-Lin Tain
Feb 25, 2018·Translational Research : the Journal of Laboratory and Clinical Medicine·Santosh L SarafVictor R Gordeuk

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Methods Mentioned

BETA
RNA-Seq
ELISA
PCR

Software Mentioned

TopHat
cuffdiff
Statistical Package for the Social Sciences ( SPSS )
CASAVA
Ensembl
DAVID
Cufflinks
GeneTool
Illumina GA Pipeline
NIH DAVID Bioinformatics Resources

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