Transcriptome analysis of air-breathing land slug, Incilaria fruhstorferi reveals functional insights into growth, immunity, and reproduction

BMC Genomics
Bharat Bhusan PatnaikYong Seok Lee

Abstract

Incilaria (= Meghimatium) fruhstorferi is an air-breathing land slug found in restricted habitats of Japan, Taiwan and selected provinces of South Korea (Jeju, Chuncheon, Busan, and Deokjeokdo). The species is on a decline due to depletion of forest cover, predation by natural enemies, and collection. To facilitate the conservation of the species, it is important to decide on a number of traits related to growth, immunity and reproduction addressing fitness advantage of the species. The visceral mass transcriptome of I. fruhstorferi was enabled using the Illumina HiSeq 4000 sequencing platform. According to BUSCO (Benchmarking Universal Single-Copy Orthologs) method, the transcriptome was considered complete with 91.8% of ortholog genes present (Single: 70.7%; Duplicated: 21.1%). A total of 96.79% of the raw read sequences were processed as clean reads. TransDecoder identified 197,271 contigs that contained candidate-coding regions. Of a total of 50,230 unigenes, 34,470 (68.62% of the total unigenes) annotated to homologous proteins in the Protostome database (PANM-DB). The GO term and KEGG pathway analysis indicated genes involved in metabolism, phosphatidylinositol signalling system, aminobenzoate degradation, and T-cell rece...Continue Reading

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Datasets Mentioned

BETA
AHA83602.1
BAK19511
AET79206
EKC34473
AHI17285
AAH46009.1
AK062848.1

Methods Mentioned

BETA
PCR
Illumina sequencing
environmental stress

Software Mentioned

est
TGICL TIGR gene indices
ORFPredictor
PSIPRED
ClustalX2
DNASTAR LASERGENE
Repeatmasker
InterProScan
IfTollip
BLAST2GO

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