Transcriptome analysis of alcohol-treated microglia reveals downregulation of beta amyloid phagocytosis

Journal of Neuroinflammation
Sergey KalininDouglas L Feinstein

Abstract

Microglial activation contributes to the neuropathology associated with chronic alcohol exposure and withdrawal, including the expression of inflammatory and anti-inflammatory genes. In the current study, we examined the transcriptome of primary rat microglial cells following incubation with alcohol alone, or alcohol together with a robust inflammatory stimulus. Primary microglia were prepared from mixed rat glial cultures. Cells were incubated with 75 mM ethanol alone or with proinflammatory cytokines ("TII": IL1β, IFNγ, and TNFα). Isolated mRNA was used for RNAseq analysis and qPCR. Effects of alcohol on phagocytosis were determined by uptake of oligomeric amyloid beta. Alcohol induced nitrite production in control cells and increased nitrite production in cells co-treated with TII. RNAseq analysis of microglia exposed for 24 h to alcohol identified 312 differentially expressed mRNAs ("Alc-DEs"), with changes confirmed by qPCR analysis. Gene ontology analysis identified phagosome as one of the highest-ranking KEGG pathways including transcripts regulating phagocytosis. Alcohol also increased several complement-related mRNAs that have roles in phagocytosis, including C1qa, b, and c; C3; and C3aR1. RNAseq analysis identified ov...Continue Reading

References

Jan 5, 2001·Alcoholism, Clinical and Experimental Research·J M LewohlR A Harris
Jun 18, 2002·Journal of Neurochemistry·R Dayne MayfieldR Adron Harris
Jun 5, 2003·Molecular Neurobiology·Hiroshi Nakanishi
Apr 7, 2004·Journal of the American Geriatrics Society·Jose A LuchsingerRichard Mayeux
Apr 20, 2004·Neuroreport·Ana M BlancoConsuelo Guerri
Jun 11, 2005·Journal of Neuroscience Research·Jose L M MadrigalCinzia Dello Russo
Sep 24, 2005·Clinical and Experimental Immunology·M R MorrisM B Hallett
Oct 28, 2005·Nature Neuroscience·David M Lovinger, John C Crabbe
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ana M BlancoConsuelo Guerri
Nov 18, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jianwen LiuR Dayne Mayfield
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Thierry GustotOlivier Le Moine
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Ana María Blanco, Consuelo Guerri
Apr 25, 2007·Alcoholism, Clinical and Experimental Research·Lucinda G CarrHoward J Edenberg
Dec 7, 2007·Molecular Immunology·Sara Fernandez-LizarbeConsuelo Guerri
Apr 29, 2008·Journal of Neuroscience Research·Silvia ParadisiMichele Mazzanti
Jun 20, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Boris TabakoffPaula L Hoffman
Mar 28, 2009·American Journal of Respiratory and Critical Care Medicine·Andrew Conway MorrisA John Simpson
Sep 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara Fernandez-LizarbeConsuelo Guerri
Nov 7, 2009·The American Journal of Pathology·Sadanand GaikwadHarald Neumann
Mar 5, 2010·Fundamental & Clinical Pharmacology·Nada Choucair-JaafarJean-Pierre Gies
Jun 18, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Silvia Alfonso-LoechesConsuelo Guerri
Mar 4, 2011·Age and Ageing·Siegfried WeyererUNKNOWN German AgeCoDe Study group (German Study on Ageing, Cognition and Dementia in Primary Care Patients)
Mar 15, 2011·Brain, Behavior, and Immunity·R G AgrawalS E Bergeson
Sep 1, 2011·Journal of Leukocyte Biology·John Karavitis, Elizabeth J Kovacs
Oct 1, 2011·British Journal of Pharmacology·Subhash C Pandey
Mar 1, 2012·The Biochemical Journal·Keefe T ChanJames E Bear
Jul 20, 2012·BMC Systems Biology·Zhongxue ChenYouping Deng
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Nov 23, 2012·Aging & Mental Health·Anna K Piazza-GardnerAdam E Barry
Mar 20, 2013·Molecular Aspects of Medicine·Nicolas MontalbettiMatthias A Hediger
Apr 23, 2013·Journal of Neurochemistry·Sara Fernandez-LizarbeConsuelo Guerri
May 24, 2013·Autophagy·Ying Sun, Gregory A Grabowski

❮ Previous
Next ❯

Citations

Aug 26, 2020·Journal of Neurotrauma·Zachary F StielperNicholas W Gilpin
Aug 23, 2020·Molecular Neurobiology·Archna SharmaPing Wang
May 9, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sunitha KodidelaSantosh Kumar
Jun 29, 2021·Frontiers in Molecular Biosciences·Iskra AraujoDanielle Gulick
Nov 30, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Xiangqian LiuSulie L Chang

❮ Previous
Next ❯

Methods Mentioned

BETA
sedation
acetylation
RNAseq
FCS
flow cytometry
PCR
reverse-transcription
transgenic

Software Mentioned

Deseq2
Flowing
DAVID
Gallios
GO Consortium
STAR aligner

Related Concepts

Related Feeds

Alzheimer's Disease: Transcription

Impaired transcription is associated with the pathogenesis and progression of conditions such as Alzheimer's disease (AD). Here are the latest discoveries pertaining to transcription and AD.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.