Transcriptome Analysis of Brassica rapa Near-Isogenic Lines Carrying Clubroot-Resistant and -Susceptible Alleles in Response to Plasmodiophora brassicae during Early Infection

Frontiers in Plant Science
Jingjing ChenZhongyun Piao

Abstract

Although Plasmodiophora brassicae is one of the most common pathogens worldwide, the causal agent of clubroot disease in Brassica crops, resistance mechanisms to it are still only poorly understood. To study the early defense response induced by P. brassicae infection, a global transcriptome profiling of the roots of two near-isogenic lines (NILs) of clubroot-resistant (CR BJN3-2) and clubroot-susceptible (BJN3-2) Chinese cabbage (Brassica rapa) was performed by RNA-seq. Among the 42,730 unique genes mapped to the reference genome of B. rapa, 1875, and 2103 genes were found to be up- and down-regulated between CR BJN3-2 and BJN3-2, respectively, at 0, 12, 72, and 96 h after inoculation (hai). Functional annotation showed that most of the differently expressed genes are involved in metabolism, transport, signal transduction, and defense. Of the genes assigned to plant-pathogen interactions, 151 showed different expression patterns between two NILs, including genes associated with pathogen-associated molecular patterns (PAMPs) and effectors recognition, calcium ion influx, hormone signaling, pathogenesis-related (PR) genes, transcription factors, and cell wall modification. In particular, the expression level of effector receptor...Continue Reading

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Citations

Aug 6, 2020·Frontiers in Plant Science·Leonardo Galindo-GonzálezStephen E Strelkov
Sep 24, 2020·Molecular Biology and Evolution·Ana Cristina BarraganEunyoung Chae
Mar 27, 2018·Plant Science : an International Journal of Experimental Plant Biology·Jingjing ChenZhongyun Piao
Jun 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junrey AmasJacqueline Batley
Mar 3, 2021·Genome Génome / Conseil National De Recherches Canada·Jakir HasanHabibur Rahman
Nov 23, 2021·Frontiers in Microbiology·Leonardo Galindo-GonzálezStephen E Strelkov

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Datasets Mentioned

BETA
GSE74044

Methods Mentioned

BETA
genotyping
RNA-seq
PCR

Software Mentioned

WEGO
EBseq
Top GO
Primer Premier
TopHat
Blast2GO

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