Transcriptome analysis of Cd-treated switchgrass root revealed novel transcripts and the importance of HSF/HSP network in switchgrass Cd tolerance

Plant Cell Reports
Gang SongBin Xu

Abstract

Transcriptome analysis of Cd-treated switchgrass roots not only revealed novel switchgrass transcripts and gene structures but also highlighted the indispensable role of HSF/HSP network in switchgrass Cd tolerance. Switchgrass (Panicum virgatum L.), a C4 perennial tall grass, can be used for revegetation of Cd-contaminated soil. In the present study, a comparative transcriptome analysis of Cd-treated switchgrass roots was conducted. The result revealed a total of 462 novel transcripts and refined gene structures of 2337 transcripts. KEGG pathway and Gene Ontology analyses of the differentially expressed genes (DEGs) suggested that activation of redox homeostasis and oxidation-related metabolic processes were the primary response to Cd stress in switchgrass roots. In particular, 21 out of 23 differentially expressed shock transcription factor genes (HSFs), and 22 out of 23 differentially expressed heat shock protein genes (HSPs) had increased expression levels after Cd treatment. Furthermore, over-expressing one HSP-encoding gene in Arabidopsis significantly improved plant Cd tolerance. The result highlighted the activation of the redox homeostasis and the involvement of the HSF/HSP network in re-establishing normal protein conf...Continue Reading

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Citations

Oct 24, 2018·International Journal of Molecular Sciences·Madhav BhattaAlexey Morgounov
Nov 17, 2019·International Journal of Molecular Sciences·Saeed Ul HaqZhen-Hui Gong
Jun 13, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shao-Shuai BiJin-Long Li

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Methods Mentioned

BETA
RNA-seq
Illumina sequencing
PCR
electrophoresis
transgenic

Software Mentioned

R package
StringTie
Cuffcompare
pheatmap
DESeq2
CNCI
HISAT
Trimmomatic
SPSS
HMMER

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