Transcriptome analysis of dorsal root ganglia's diabetic neuropathy reveals mechanisms involved in pain and regeneration

Life Sciences
Maria Carolina Pedro AthieCarlos A Parada

Abstract

Peripheral diabetic neuropathy (DN) manifests in nearly 60% of diabetic patients, being pain its most debilitating symptom. Although electrophysiological and morphological aspects are well described, little is known about its development and progression, undermining effective therapies. Hyperglycemia and insulin signaling impairment are considered the triggering events of oxidative stress observed in the dying nerves, however there are still many gaps in the knowledge of intracellular plastic changes it generates. In this study we aimed to evaluate the early transcriptome changes in DN when the first symptoms of the disease start to appear. Next-Generation Sequencing of messenger RNA (RNA-Seq) of L4 and L5 dorsal root ganglia (DRG) four weeks post-diabetes induction in a rat model for type 1 diabetes. RNA sequencing found 66 transcripts differentially expressed between diabetic and control groups, related mainly to the following biological processes: inflammation, hyperalgesia/analgesia, cell growth and cell survival. Given their roles, the differentially expressed genes suggest an attempt to switch to a survival/regenerative program. Our results show that changes in the transcriptome profile start to appear early in the course...Continue Reading

Citations

Oct 31, 2018·Molecular Omics·H StarobovaI Vetter
Feb 20, 2021·Biochemistry·Minji JeonAvi Ma'ayan
May 25, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Nicole C NowakAmy S Paller

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