Transcriptome analysis of hen preadipocytes treated with an adipogenic cocktail (DMIOA) with or without 20(S)-hydroxylcholesterol

BMC Genomics
Alemu Regassa, Woo-Kyun Kim

Abstract

20(S)-hydroxycholesterol (20(S)) potentially reduces adipogenesis in mammalian cells. The role of this oxysterol and molecular mechanisms underlying the adipogenesis of preadipocytes from laying hens have not been investigated. This study was conducted to 1. Analyze genes differentially expressed between preadipocytes treated with an adipogenic cocktail (DMIOA) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 20 μg/mL insulin and 300 μM oleic acid (OA) and control cells and 2. Analyze genes differentially expressed between preadipocytes treated with DMIOA and those treated with DMIOA + 20(S) using Affymetrix GeneChip® Chicken Genome Arrays. In experiment one, where we compared the gene expression profile of non-treated (control) cells with those treated with DMIOA, out of 1,221 differentially expressed genes, 755 were over-expressed in control cells, and 466 were over-expressed in cells treated with DMIOA. In experiment two, where we compared the gene expression profile of DMIOA treated cells with those treated with DMIOA+20(S), out of 212 differentially expressed genes, 90 were over-expressed in cells treated with DMIOA, and 122 were over-expressed in those treated with DMIOA+20(S). Genes over-expressed in ...Continue Reading

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Citations

Jul 28, 2016·Cell Biology International·Alemu RegassaWoo Kyun Kim
Dec 2, 2017·Journal of the American Heart Association·Gabor GäbelLesca M Holdt
Sep 25, 2017·Poultry Science·Guoqing WangElizabeth R Gilbert
Jul 4, 2017·Chemistry and Physics of Lipids·Débora LevySérgio P Bydlowski

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Datasets Mentioned

BETA
GSE50880

Methods Mentioned

BETA
dissection
electrophoresis
in vitro-transcription

Software Mentioned

SAS
Affymetrix GeneChip Command Console
Ingenuity Pathways Analysis
FlexArray
IPA

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