Transcriptome analysis of leaf senescence in red clover (Trifolium pratense L.)

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Yuehui ChaoLiebao Han

Abstract

Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is used as forage. Leaf senescence is a critical developmental process that negatively affects plant quality and yield. The regulatory mechanism of leaf senescence has been studied, and genes involved in leaf senescence have been cloned and characterized in many plants. However, those works mainly focused on model plants. Information about regulatory pathways and the genes involved in leaf senescence in red clover is very sparse. In this study, to better understand leaf senescence in red clover, transcriptome analysis of mature and senescent leaves was investigated using RNA-Seq. A total of about 35,067 genes were identified, and 481 genes were differentially expressed in mature and senescent leaves. Some identified differentially expressed genes showed similar expression patterns as those involved in leaf senescence in other species, such as Arabidopsis, Medicago truncatula and rice. Differentially expressed genes were confirmed by quantitative real-time PCR (qRT-PCR). Genes involved in signal transduction, transportation and metabolism of plant hormones, transcription factors and plant senescence were upregulated, while the downregulated genes ...Continue Reading

References

Apr 1, 1992·Experimental Cell Research·C Wistrom, B Villeponteau
Apr 1, 1997·Current Opinion in Biotechnology·H G Nam
Jul 24, 1998·The Plant Journal : for Cell and Molecular Biology·M DelarueC Bellini
Mar 14, 2002·Plant Physiology·Yuehui HeSusheng Gan
Mar 26, 2002·Journal of Experimental Botany·Stefan Hörtensteiner, Urs Feller
Feb 12, 2004·The Plant Journal : for Cell and Molecular Biology·Michael Dalgaard MikkelsenBarbara Ann Halkier
Apr 28, 2006·The Plant Journal : for Cell and Molecular Biology·Yongfeng Guo, Susheng Gan
Oct 1, 1970·Plant Physiology·S Mayak, A H Halevy
Dec 1, 1980·Plant Physiology·J S Samet, T R Sinclair
Sep 22, 2006·Methods in Molecular Biology·Michael L Sullivan, Kenneth H Quesenberry
Jan 9, 2007·Plant Biotechnology Journal·Per L Gregersen, Preben Bach Holm
Jan 24, 2007·Plant Physiology·Bhadra GunesekeraGlenda E Gillaspy
Sep 20, 2008·Plant Biology·S BalazadehB Mueller-Roeber
Mar 6, 2009·Planta·Michael Dalgaard MikkelsenBarbara Ann Halkier
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Oct 2, 2009·Plant Signaling & Behavior·Roberto De MicheleFiorella Lo Schiavo
Oct 28, 2009·Nucleic Acids Research·Paulino Pérez-RodríguezBernd Mueller-Roeber
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Nov 9, 2010·Plant Signaling & Behavior·Martin A SeltmannSusanne Berger
Feb 10, 2011·Molecular Plant·Salma BalazadehBernd Mueller-Roeber
Apr 23, 2011·Journal of Experimental Botany·Jeong Im KimMeena L Narasimhan
Oct 20, 2011·The Plant Journal : for Cell and Molecular Biology·Kewei ZhangSu-Sheng Gan

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
RNA-Seq
MDA
RNA Assay
PCR
Illumina sequencing

Software Mentioned

BLASTx
KOBAS
GOseq R package
DESeq R package
Bowtie
TopHat
HTSeq

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.