Transcriptome analysis of Liriomyza trifolii (Diptera: Agromyzidae) in response to temperature stress.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Ya-Wen ChangYu-Zhou Du

Abstract

The leafminer Liriomyza trifolii is an important insect pest of ornamental and vegetable crops worldwide. Temperature is a critical environmental factor that impacts both the distribution and interspecific competition of Liriomyza spp. In this study, we compared the transcriptomes of L. trifolii exposed to ambient (25 °C), hot (43 °C), and cold (-7 °C) temperatures. RNA-seq revealed 100,041 assembled unigenes, and 50,546 of these were annotated in L. trifolii transcriptome libraries. A total of 207 and 2904 differentially expressed genes (DEGs) were identified in response to hot and cold stress, respectively. Functional classification indicated that "cellular process", "single organism processes" and "metabolic processes" pathways were significantly enriched, along with "binding activity" and "catalytic activity". With respect to clusters of orthologous genes (COG) classification, DEGs were assigned to "post-translational modification, protein turnover, chaperones", "carbohydrate transport and metabolism" and "lipid transport and metabolism" categories. Subsequent annotation and enrichment analyses indicated that genes encoding heat shock proteins (HSPs) and cuticular proteins were significantly up-regulated during heat and col...Continue Reading

References

Jan 7, 1998·Journal of Applied Physiology·P L Moseley
Feb 22, 2001·Annual Review of Physiology·J G Duman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 31, 2007·Journal of Biosciences·Richa AryaSubhash C Lakhotia
Oct 1, 1948·Biological Reviews of the Cambridge Philosophical Society·V B WIGGLESWORTH
Aug 16, 2008·Journal of Insect Physiology·Jelena PuraćMelody S Clark
Aug 20, 2008·Annual Review of Entomology·Le KangTong-Xian Liu
Sep 4, 2009·Annual Review of Entomology·Estela L Arrese, Jose L Soulages
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Dec 20, 2013·The Journal of Experimental Biology·Nicholas M Teets, David L Denlinger
Nov 4, 2015·Annual Review of Entomology·Fang-Hao Wan, Nian-Wan Yang
Nov 23, 2015·Journal of Thermal Biology·Brent J SinclairLaura V Ferguson
Apr 20, 2017·Pest Management Science·Yulin GaoZhongren Lei

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Citations

Jul 25, 2021·Journal of Insect Physiology·Kathryn M ChipchaseKathleen A Killian
Aug 21, 2021·Molecular Ecology·Rémy PerezSerge Aron

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