Transcriptome Analysis of Murine Olfactory Sensory Neurons during Development Using Single Cell RNA-Seq

Chemical Senses
Paul ScholzSabrina Osterloh

Abstract

Mammalian odor reception is achieved by highly specialized olfactory sensory neurons (OSNs) located in the nasal cavity. Despite their importance for the daily survival of most mammals, the gene expression and regulatory profiles of these single neurons are poorly understood. Here, we report the isolation of individual GFP-labeled OSNs from Olfr73-GFP mice at different developmental stages followed by Next Generation Sequencing, thereby analyzing the detailed transcriptome for the first time. We characterized the repertoire of olfactory receptors (ORs) and found that in addition to the highly and predominant detectable Olfr73, 20 additional ORs were stably detectable at lower transcript levels in adult mice. Additionally, OSNs collected from mice of earlier developmental stages did not show any stable OR patterns. However, more than one predominant OR per OSN was detectable.

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Citations

Apr 12, 2016·Frontiers in Cellular Neuroscience·Paul ScholzSabrina Osterloh
Aug 16, 2016·BMC Genomics·Tsviya OlenderDoron Lancet
Sep 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jung-Mi ChoiHaeyoung Suh-Kim
May 20, 2017·Chemical Senses·Qiang WangTimothy S McClintock
Jun 10, 2017·The Journal of Comparative Neurology·Julie H ColemanJames E Schwob
Mar 9, 2017·Frontiers in Genetics·Andrea Degl'Innocenti, Anna D'Errico
Aug 31, 2018·Frontiers in Cell and Developmental Biology·Chrissy KandelKlaus Deckmann
May 26, 2018·Chemical Senses·Longzhi Tan, Xiaoliang Sunney Xie
Dec 29, 2020·Molecular and Cellular Neurosciences·Elizabeth A CoreyBarry W Ache
Oct 8, 2020·Cell Reports·Carl J van der LindenStephen W Santoro

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