Transcriptome analysis of northern elephant seal (Mirounga angustirostris) muscle tissue provides a novel molecular resource and physiological insights

BMC Genomics
Jane I KhudyakovDaniel E Crocker

Abstract

The northern elephant seal, Mirounga angustirostris, is a valuable animal model of fasting adaptation and hypoxic stress tolerance. However, no reference sequence is currently available for this and many other marine mammal study systems, hindering molecular understanding of marine adaptations and unique physiology. We sequenced a transcriptome of M. angustirostris derived from muscle sampled during an acute stress challenge experiment to identify species-specific markers of stress axis activation and recovery. De novo assembly generated 164,966 contigs and a total of 522,699 transcripts, of which 68.70% were annotated using mouse, human, and domestic dog reference protein sequences. To reduce transcript redundancy, we removed highly similar isoforms in large gene families and produced a filtered assembly containing 336,657 transcripts. We found that a large number of annotated genes are associated with metabolic signaling, immune and stress responses, and muscle function. Preliminary differential expression analysis suggests a limited transcriptional response to acute stress involving alterations in metabolic and immune pathways and muscle tissue maintenance, potentially driven by early response transcription factors such as C...Continue Reading

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Citations

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Jul 15, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Julia María Torres-VelardeJosé Pablo Vázquez-Medina

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Datasets Mentioned

BETA
SRP045540

Methods Mentioned

BETA
sedation
biopsy
PCR

Software Mentioned

BLASTX
TBLASTN
FastQC
bowtie
HIT
Fastx
Trimmomatic
samtools
Illumina Casava
Bioconductor

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