Transcriptome Analysis of Poplar Under Salt Stress and Over-Expression of Transcription Factor NAC57 Gene Confers Salt Tolerance in Transgenic Arabidopsis

Frontiers in Plant Science
Wenjing YaoTingbo Jiang

Abstract

NAC domain genes belong to a large plant-specific transcription factor family, which is well-known to be associated with multiple stress responses and plant developmental processes. In this study, we screened differentially expressed genes (DEGs) and detected mRNA abundance of NAC family by RNA-Seq in the poplar leaves under salt stress condition. A total of 276 up-regulated DEGs and 159 down-regulated DEGs were identified to be shared in Populus alba × Populus glandulosa and Populus simonii × Populus nigra. Among 170 NAC members, NAC57 gene was significantly up-regulated in response to salt stress in the two species. Tissue-specific and salt-responsive analyses indicated the expression pattern of NAC57 gene was spatial and temporal in poplar under salt stress. Particle bombardment results showed subcellular localization of NAC57 was not solely nucleus-targeted. Full-length cDNA sequence of the NAC57 gene was cloned from P. alba × P. glandulosa and transformed into Arabidopsis thaliana. Under salt stress, transgenic Arabidopsis overexpressing NAC57 showed higher seed germination rate, root length, and fresh weight than wild type plants. In addition, the transgenic plants displayed higher superoxide dismutase activity and peroxi...Continue Reading

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Citations

Nov 22, 2019·Frontiers in Plant Science·Zihan ChengTingbo Jiang
Jan 10, 2020·BMC Plant Biology·Wenjing YaoTingbo Jiang
Jun 18, 2020·Frontiers in Plant Science·Lei ZhangJianjun Hu
Apr 4, 2021·International Journal of Molecular Sciences·Xuemei ZhangTingbo Jiang
May 12, 2021·Physiologia Plantarum·Gabriella FrosiMauro Guida Santos

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Methods Mentioned

BETA
environmental stress
transgenic
RNA-Seq
RNASeq
fluorescence microscopy
MDA

Software Mentioned

Pop
Fastqc
s Pipes
Pipes
Poplar Gene Expression Data Analysis Pipelines
Gene Cluster
BLASTP
Clustal
Clustal W
MEGA

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