Transcriptome analysis of sheep oral mucosa response to Orf virus infection

PloS One
Huaijie JiaZhizhong Jing

Abstract

Contagious ecthyma is a highly contagious disease with worldwide distribution, which is caused by the Orf virus (ORFV) belonging to the Parapoxvirus. To study the alteration of host gene expression in response to ORFV infection at the transcriptional level, several young small-tailed Han sheep were inoculated with ORFV, and their oral mucosa tissue samples (T0, T3, T7 and T15) were collected on day 0, 3, 7 and 15 after ORFV infection respectively. RNA-seq transcriptome comparisons were performed, showing that 1928, 3219 and 2646 differentially expressed genes (DEGs) were identified among T3 vs. T0, T7 vs. T0, and T15 vs. T0 respectively. Gene Ontology (GO) analyses of the DEGs from these comparisons, revealed that ORFV might provoke vigorous immune response of the host cells during the early stage of infection. Moreover, GO and network analysis showed that positive and negative regulative mechanisms of apoptosis were integrated in the host cells through up or down-regulating the expression level of DEGs involved in apoptotic pathways, in order to reach a homeostasis of oral mucosa tissues during the exposure to ORFV infection. In conclusion, our study for the first time describes the direct effects of ORFV on the global host ge...Continue Reading

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Citations

Oct 30, 2018·Reviews in Medical Virology·Ruixue WangShuhong Luo
Aug 9, 2020·Microbiology Resource Announcements·Daniel L HeareSara D Lawhon
Nov 12, 2020·The Biochemical Journal·Chathura D SuraweeraMarc Kvansakul
Jan 28, 2021·Virology Journal·Guohua ChenZhizhong Jing

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Datasets Mentioned

BETA
GSE95203

Methods Mentioned

BETA
RNA-seq
electrophoresis
PCR

Software Mentioned

Cytoscape
DAVID
edgeR
TopHat

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