Transcriptome Analysis of the Inhibitory Effect of Astaxanthin on Helicobacter pylori-Induced Gastric Carcinoma Cell Motility.

Marine Drugs
Suhn Hyung Kim, Hyeyoung Kim

Abstract

Helicobacter pylori (H. pylori) infection promotes the metastasis of gastric carcinoma cells by modulating signal transduction pathways that regulate cell proliferation, motility, and invasion. Astaxanthin (ASTX), a xanthophyll carotenoid, is known to inhibit cancer cell migration and invasion, however the mechanism of action of ASTX in H. pylori-infected gastric epithelial cells is not well understood. To gain insight into this process, we carried out a comparative RNA sequencing (RNA-Seq) analysis of human gastric cancer AGS (adenocarcinoma gastric) cells as a function of H. pylori infection and ASTX administration. The results were used to identify genes that are differently expressed in response to H. pylori and ASTX. Gene ontology (GO) analysis identified differentially expressed genes (DEGs) to be associated with cell cytoskeleton remodeling, motility, and/or migration. Among the 20 genes identified, those encoding c-MET, PI3KC2, PLCγ1, Cdc42, and ROCK1 were selected for verification by real-time PCR analysis. The verified genes were mapped, using signaling networks contained in the KEGG database, to create a signaling pathway through which ASTX might mitigate the effects of H. pylori-infection. We propose that H. pylori-...Continue Reading

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Citations

Sep 2, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Seyede Nazanin ZarneshanLuciano Saso

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Methods Mentioned

BETA
GTPases
PCR
RNA-seq
cDNA

Software Mentioned

ExDEGA ( Excel - based Differentially Expressed Gene Analysis )
ExDEGA
Torrent Suite
ampliSeq RNA Plug in

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