Transcriptome Analysis of the Preterm Rabbit Lung after Seven Days of Hyperoxic Exposure

PloS One
Thomas SalaetsJaan Toelen

Abstract

The neonatal management of preterm born infants often results in damage to the developing lung and subsequent morbidity, referred to as bronchopulmonary dysplasia (BPD). Animal models may help in understanding the molecular processes involved in this condition and define therapeutic targets. Our goal was to identify molecular pathways using the earlier described preterm rabbit model of hyperoxia induced lung-injury. Transcriptome analysis by mRNA-sequencing was performed on lungs from preterm rabbit pups born at day 28 of gestation (term: 31 days) and kept in hyperoxia (95% O2) for 7 days. Controls were preterm pups kept in normoxia. Transcriptomic data were analyzed using Array Studio and Ingenuity Pathway Analysis (IPA), in order to identify the central molecules responsible for the observed transcriptional changes. We detected 2217 significantly dysregulated transcripts following hyperoxia, of which 90% could be identified. Major pathophysiological dysregulations were found in inflammation, lung development, vascular development and reactive oxygen species (ROS) metabolism. To conclude, amongst the many dysregulated transcripts, major changes were found in the inflammatory, oxidative stress and lung developmental pathways. T...Continue Reading

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Mar 27, 2021·Frontiers in Medicine·Luciano AmarelleLeonel Malacrida

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Methods Mentioned

BETA
cesarean section

Software Mentioned

IPA
IPA ( Upstream Regulator Analysis and Network Generation Algor...
Ingenuity Pathway Analysis ( IPA )

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