Transcriptome analysis of the white body of the squid Euprymna tasmanica with emphasis on immune and hematopoietic gene discovery

PloS One
Karla A SalazarMaria G Castillo

Abstract

In the mutualistic relationship between the squid Euprymna tasmanica and the bioluminescent bacterium Vibrio fischeri, several host factors, including immune-related proteins, are known to interact and respond specifically and exclusively to the presence of the symbiont. In squid and octopus, the white body is considered to be an immune organ mainly due to the fact that blood cells, or hemocytes, are known to be present in high numbers and in different developmental stages. Hence, the white body has been described as the site of hematopoiesis in cephalopods. However, to our knowledge, there are no studies showing any molecular evidence of such functions. In this study, we performed a transcriptomic analysis of white body tissue of the Southern dumpling squid, E. tasmanica. Our primary goal was to gain insights into the functions of this tissue and to test for the presence of gene transcripts associated with hematopoietic and immune processes. Several hematopoiesis genes including CPSF1, GATA 2, TFIID, and FGFR2 were found to be expressed in the white body. In addition, transcripts associated with immune-related signal transduction pathways, such as the toll-like receptor/NF-κβ, and MAPK pathways were also found, as well as othe...Continue Reading

References

Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Sottrup-JensenA Lundwall
Nov 18, 1997·Cell·R Medzhitov, C A Janeway
Oct 29, 2000·British Journal of Haematology·A ScaccabarozziS Fargion
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D S KaufmanJ A Thomson
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
May 3, 2002·Blood·Frank M Torti, Suzy V Torti
May 29, 2002·Oncogene·Jiang Zhu, Stephen G Emerson
Dec 24, 2002·The Journal of Biological Chemistry·Peter MellrothHakan Steiner
Jun 12, 2003·Annals of the New York Academy of Sciences·Christina GiesertHans-Jorg Buhring
Sep 25, 2003·The Journal of Biological Chemistry·Zheng-Ming WangRoman Dziarski
Dec 31, 2003·Molecular Immunology·Masaru Nonaka, Fumiko Yoshizaki
May 4, 2004·Molecular and Cellular Biology·Jesús GilMariano Esteban
Sep 10, 2004·The Journal of Biological Chemistry·Floris J BikkerJan Mollenhauer
Nov 2, 2004·Nature Reviews. Immunology·Kingston H G Mills
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rongjin GuanRoy A Mariuzza
Feb 19, 2005·Science·Joseph T OpfermanStanley J Korsmeyer
Mar 26, 2005·Science·Fredrik BäckhedJeffrey I Gordon
Jul 26, 2005·Immunogenetics·Larry J DishawCharles H Bigger
Nov 5, 2005·Applied and Environmental Microbiology·Michael S GoodsonMargaret J McFall-Ngai
Feb 16, 2007·The Biological Bulletin·Tanya A KoropatnickMargaret J McFall-Ngai
Mar 14, 2007·PLoS Pathogens·Linh N PhamDavid S Schneider
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J Williams
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip RosenstielStefan Schreiber
Oct 16, 2007·Oncogene·H Iwasaki, K Akashi
Jun 17, 2008·Current Opinion in Microbiology·Brian P Lazzaro
Jun 20, 2008·Cellular Microbiology·Carlos J RosadoMichelle A Dunstone
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zachary J KrausGail A Bishop
Feb 7, 2009·Environmental Microbiology·Spencer V NyholmMargaret J McFall-Ngai
Feb 19, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Chris G CarterNatalie A Moltschaniwskyj
Sep 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniel O HartMichael R Green

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Citations

Nov 26, 2015·Developmental and Comparative Immunology·E A PilaP C Hanington
Jun 29, 2015·Fish & Shellfish Immunology·Maria G CastilloNina R Joffe
Mar 23, 2017·Developmental and Comparative Immunology·Jonathan H Schultz, Coen M Adema
Jan 31, 2019·Frontiers in Physiology·Laura López-GalindoClara Galindo-Sánchez
Jun 6, 2021·Nature Reviews. Microbiology·Spencer V Nyholm, Margaret J McFall-Ngai

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Datasets Mentioned

BETA
SRP049997
AFV94409

Methods Mentioned

BETA
dissection
chips
SMA

Software Mentioned

gsAssembler Newbler
BLAST
InterProScan
WU
BLASTx
DESeq
Blast2GO
ClustalX

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