Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production

Scientific Reports
Yoshihiko OndaKikukatsu Ito

Abstract

Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-D-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP ...Continue Reading

References

Aug 23, 1991·Biochimica Et Biophysica Acta·A L Moore, J N Siedow
Jul 1, 1994·Plant Physiology·L McIntosh
Aug 1, 1995·Journal of Bioenergetics and Biomembranes·D A Day, J T Wiskich
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 20, 1997·Nature·M LaloiD Ricquier
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·L M LoisA Boronat
Jul 1, 1995·The Plant Cell·J. N. Siedow, A. L. Umbach
Dec 13, 2002·Nature·Marcus C StensmyrAnna-Maria Angioy
Nov 25, 2003·Nature·Roger S SeymourMarc Gibernau
Feb 24, 2005·Annual Review of Plant Biology·Allan G RasmussonThomas E Elthon
Jul 1, 1992·Plant Physiology·I Raskin
May 4, 2006·Annual Review of Plant Biology·Aníbal Eugênio VercesiHernan Chaimovich
May 30, 2006·Biochimica Et Biophysica Acta·Richard K Porter
Dec 15, 1972·Science·K A NagyR S Seymour
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 30, 2009·Plant, Cell & Environment·Roger S SeymourStergios Arg Pirintsos
Jul 9, 2009·Journal of Experimental Botany·Elizabeth CordobaPatricia León
Mar 23, 2011·Phytochemistry·Isabella UrruBill S Hansson
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Sep 8, 2011·Nature Reviews. Genetics·Jeffrey A Martin, Zhong Wang
Oct 25, 2011·Annals of Botany·Pavel VeselýTomás Pavlícek
Dec 6, 2011·Nucleic Acids Research·Philippe LameschEva Huala
Feb 4, 2012·American Journal of Botany·Judson A WardCourtney A Weber
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·John P Hamilton, C Robin Buell

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Citations

Aug 17, 2016·Scientific Reports·Zhonglai LuoDianxiang Zhang
Nov 1, 2018·Functional Plant Biology : FPB·Danae LainaStergios A Pirintsos
May 14, 2020·International Journal of Molecular Sciences·Yu ZouYi Ding
Nov 28, 2020·Journal of Experimental Botany·Vasily N PopovAbir U Igamberdiev

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Datasets Mentioned

BETA
AB485994
DRA002584

Methods Mentioned

BETA
RNA-seq

Software Mentioned

BLASTx
FASTX
eXpress
R package
KmerGenie
InterProScan
Rnnotator
BLAST2GO
DEGseq
bowtie

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