Transcriptome analysis reveals a stress response of Shewanella oneidensis deprived of background levels of ionizing radiation

PloS One
Hugo CastilloGeoffrey B Smith

Abstract

Natural ionizing background radiation has exerted a constant pressure on organisms since the first forms of life appeared on Earth, so that cells have developed molecular mechanisms to avoid or repair damages caused directly by radiation or indirectly by radiation-induced reactive oxygen species (ROS). In the present study, we investigated the transcriptional effect of depriving Shewanella oneidensis cultures of background levels of radiation by growing the cells in a mine 655 m underground, thus reducing the dose rate from 72.1 to 0.9 nGy h-1 from control to treatment, respectively. RNASeq transcriptome analysis showed the differential expression of 4.6 and 7.6% of the S. oneidensis genome during early- and late-exponential phases of growth, respectively. The greatest change observed in the treatment was the downregulation of ribosomal proteins (21% of all annotated ribosomal protein genes during early- and 14% during late-exponential) and tRNA genes (14% of all annotated tRNA genes in early-exponential), indicating a marked decrease in protein translation. Other significant changes were the upregulation of membrane transporters, implying an increase in the traffic of substrates across the cell membrane, as well as the up and ...Continue Reading

References

Jun 1, 1991·Biochimie·K H Nierhaus
Jan 1, 1984·Annual Review of Biochemistry·M NomuraG Baughman
Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Jan 1, 1994·International Journal of Radiation Biology·P A Riley
Jan 1, 1995·Annual Review of Microbiology·E B Newman, R Lin
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A Jelinsky, L D Samson
Oct 9, 2002·Nature Biotechnology·John F HeidelbergClaire M Fraser
Nov 7, 2002·Molecular Cell·Thomas M WendrichKnud H Nierhaus
May 6, 2003·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·M C Thorne
Apr 22, 2004·Molecular Cell·Shun Jin Lee, Jay D Gralla
Nov 2, 2004·Journal of Bacteriology·Haichun GaoJizhong Zhou
Jan 18, 2005·Cell·Seyedtaghi TakyarHarry F Noller
Jul 19, 2005·Photochemistry and Photobiology·Xiaoyun QiuGeorge W Sundin
Dec 27, 2005·Nucleic Acids Research·Javier Cabello-Villegas, Edward P Nikonowicz
Mar 10, 2006·Molecular & Cellular Proteomics : MCP·Steven D BrownDorothea K Thompson
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Nov 28, 2007·Journal of Bacteriology·Tim DurfeeDing Jun Jin
Jan 19, 2008·The FEBS Journal·Hiroyuki AokiM Clelia Ganoza
Sep 30, 2008·Journal of Bacteriology·Alexey E KazakovMikhail S Gelfand
Jan 7, 2010·Biochemistry·Henry Jay FormanFulvio Ursini
Jan 25, 2011·Microbial Biotechnology·Haichun GaoJizhong Zhou
Apr 5, 2011·Cell Metabolism·Michael P MurphyChristine C Winterbourn
May 20, 2011·Health Physics·Geoffrey Battle SmithRaymond A Guilmette
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Jan 31, 2012·Cellular Signalling·Paul D RayYoshiaki Tsuji
Jun 29, 2012·Journal of Radiation Research·Masanobu KawanishiTakashi Yagi

❮ Previous
Next ❯

Citations

Jan 2, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Shizuyo Sutou
Nov 18, 2020·Journal of Bacteriology·Alba Chavez-DozalMichele K Nishiguchi
Dec 29, 2020·Frontiers in Public Health·Giuseppe EspositoPatrizia Morciano
May 25, 2021·Frontiers in Genetics·Hugo CastilloGeoffrey B Smith
Aug 18, 2021·Radiation Protection Dosimetry·Konnor J KennedyDouglas R Boreham
Nov 10, 2021·Radiation and Environmental Biophysics·Hugo CastilloGeoffrey Smith

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA396034

Methods Mentioned

BETA
protein folding
Assay
PCR
RNASeq
DNA
PCA
GTPase

Software Mentioned

REVIGO
RNASeq
FASTA
edgeR
pro
EDGE

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.